Last data update: 22 May 2023 13:52 CEST
Host name: Escherichia coli K12 MG1655 SEGA016 (LMBP 12852)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | MG1655 SEGA016 |
| Accession number: | LMBP 12852 |
| Depositor: | A. Ehrmann1 and Prof. Dr M. Nørholm1. It was constructed by C. Bayer1, A. Ehrmann1 and M. Rennig1. 1 The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Horsholm, Denmark |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Other culture collection numbers: | SEGA 016 |
| Medium: | LB-Lennox |
| Selection marker: | tetracycline (10 µg/ml) |
| Temperature: | 37°C |
| Genotype: | F- λ- ilvG- rfb-50 rph-1 glmS-pstS::J23100-high-PROTi-gfp-tetA (Source: http://openwetware.org/wiki/E._coli_genotypes#MG1655 + Bayer et al., 2021) |
| SEGA sequence: | p12852.gb |
| Phenotype: | tetR |
| Properties: | The strain was created based on Escherichia coli K12 MG1655, by integrating the SEGA landing pad between the glmS and pstS loci. Counter-selection is posssible through sensitivity to NiCl2 (50 µM). |
| Further information: | This host strain is intended for simplistic bacterial genome engineering, using the SEGA genome architecture. |
| Original reference: | Bayer et al., Nat. Commun. 12 (2021), 5876 [PMID: 34620865] [DOI: 10.1038/s41467-021-26155-5] |
| Restricted use: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department. |
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Refer in your Materials and Methods: |
Escherichia coli K12 MG1655 SEGA016 (LMBP 12852) is available at BCCM/GeneCorner. It was deposited by A. Ehrmann and Prof. Dr M. Nørholm and was published in Bayer et al., 2021. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.