GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 MG1655 SEGA097 (LMBP 12933)

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Species:Escherichia coli
Strain designation:MG1655 SEGA097
Accession number: LMBP 12933
Depositor: A. Ehrmann1 and Prof. Dr M. Nørholm1. It was constructed by C. Bayer1, A. Ehrmann1 and M. Rennig1.
1 The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Horsholm, Denmark
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Other culture collection numbers:SEGA 097
Medium: LB-Lennox
Selection marker: -
Temperature: 37°C
Genotype: F- λ- ilvG- rfb-50 rph-1 glmS-pstS::J23100-high-BCD-3'tetA (Source: + Bayer et al., 2021)
Phenotype: tetS
Properties: The strain was created based on Escherichia coli K12 MG1655, by integrating the SEGA landing pad between the glmS and pstS loci.
Further information: This host strain is intended for simplistic bacterial genome engineering, using the SEGA genome architecture.
Original reference: Bayer et al., Nat. Commun. 12 (2021), 5876 [PMID: 34620865] [DOI: 10.1038/s41467-021-26155-5]
Restricted use: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.

Refer in your Materials and Methods:

Escherichia coli K12 MG1655 SEGA097 (LMBP 12933) is available at BCCM/GeneCorner. It was deposited by A. Ehrmann and Prof. Dr M. Nørholm and was published in Bayer et al., 2021.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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