GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli B Evo21(DE3) (LMBP 12941)

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Species:Escherichia coli
Strain designation:Evo21(DE3)
Accession number: LMBP 12941
Depositor: Dr S. Heyde1 and Prof. Dr M. Nørholm1. It was constructed by Dr S. Heyde1.
1 The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Horsholm, Denmark
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Lennox
Selection marker: -
Temperature: 28°C
Genotype: F- ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7gene1 ind1 sam7 nin5]) ΔlacI ΔlacZ rne::IS1
Phenotype: -
Properties: This strain is intended for protein production. The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. The lacI and lacZ genes have been deleted.
Further information: The genome sequence of this strain corresponds with EMBL project number PRJNA741995.
Original reference: Heyde et al., Commun. Biol. 4 (2021), 963 [PMID: 34385596] [DOI: 10.1038/s42003-021-02493-4]
Restricted use: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.

Refer in your Materials and Methods:

Escherichia coli B Evo21(DE3) (LMBP 12941) is available at BCCM/GeneCorner. It was deposited by Dr S. Heyde and Prof. Dr M. Nørholm and was published in Heyde et al., 2021.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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