Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 M5219 (LMBP 130)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | M5219 |
| Accession number: | LMBP 130 |
| Depositor: | Prof. E. Remaut1 2 1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium 2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Lennox |
| Selection marker: | / |
| Temperature: | 28°C |
| Genotype: | lacZam trpAam rpsL λ(bio252 cI857 ΔH1) |
| Phenotype: | StrR |
| Properties: | Expression host for plasmids containing phage λ's PL or PR promoter. Induction is obtained by shifting the culture from 28°C to 42°C. The resident λ is a defective lysogen. ΔH1 removes part of cro and all genes to the right of it. bio252 removes all genes to the left of cIII. cI857 is ind-. At 42°C, N is expressed from the lysogen. As a consequence, transcription termination signals that might be present downstream from the PL promoter and the nutL site, as present on the pPL-series of plasmids, can be overridden. |
| Further information: | Useful for the expression of cloned genes carrying transcription terminators between the start of the gene and the insertion point. |
| Original reference: | Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli K12 M5219 (LMBP 130) is available at BCCM/GeneCorner. It was deposited by Prof. E. Remaut and was published in Remaut et al., 1981. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.