GeneCorner host details

Last data update: 20 September 2021 09:16 CEST

Host name: Escherichia coli K12 M5219 (LMBP 130)

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Species:Escherichia coli
Strain designation:M5219
Accession number: LMBP 130
Depositor: Prof. E. Remaut1 2
1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Lennox
Selection marker: /
Temperature: 28°C
Genotype: lacZam trpAam rpsL λ(bio252 cI857 ΔH1)
Phenotype: StrR
Properties: Expression host for plasmids containing phage λ's PL or PR promoter. Induction is obtained by shifting the culture from 28°C to 42°C. The resident λ is a defective lysogen. ΔH1 removes part of cro and all genes to the right of it. bio252 removes all genes to the left of cIII. cI857 is ind-. At 42°C, N is expressed from the lysogen. As a consequence, transcription termination signals that might be present downstream from the PL promoter and the nutL site, as present on the pPL-series of plasmids, can be overridden.
Further information: Useful for the expression of cloned genes carrying transcription terminators between the start of the gene and the insertion point.
Original reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 M5219 (LMBP 130) is available at BCCM/GeneCorner. It was deposited by Prof. E. Remaut and was published in Remaut et al., 1981.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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