Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 BW313 (LMBP 1325)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | BW313 |
| Accession number: | LMBP 1325 |
| Depositor: | Prof. Dr E. Remaut1 2 1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium 2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Other culture collection numbers: | CGSC 6796 |
| Medium: | LB-Lennox |
| Selection marker: | / |
| Temperature: | 37°C |
| Genotype: | λ- ung-1 relA1 dut-1 spoT1 thiE1 (Source: CGSC 6796) |
| Phenotype: | - |
| Properties: | Use of this strain is required for the preparation of single-stranded phage M13 or phasmid DNA to be used in the site-directed mutagenesis method of Kunkel (Kunkel, 1985). |
| Further information: | - |
| Original reference: | Kunkel, Proc. Natl. Acad. Sci. U.S.A. 82 (1985), 488-492 [PMID: 3881765] |
| Related reference: | Warner et al., J. Bacteriol. 145 (1981), 687-695 [PMID: 6109711] [DOI: 10.1128/jb.145.2.687-695.1981] Duncan and Weiss, J. Bacteriol. 151 (1982), 750-755 [PMID: 7047496] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli K12 BW313 (LMBP 1325) is available at BCCM/GeneCorner. It was deposited by Prof. Dr E. Remaut and was published in Kunkel, 1985. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.