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GeneCorner host details

Last data update: 20 September 2021 09:16 CEST

Host name: Escherichia coli K12 BW313 (LMBP 1325)

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Species:Escherichia coli
Group:K12
Strain designation:BW313
Accession number: LMBP 1325
Depositor: Prof. Dr E. Remaut1 2
1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Other culture collection numbers:CGSC 6796
Medium: LB-Lennox
Selection marker: /
Temperature: 37°C
Genotype: λ- ung-1 relA1 dut-1 spoT1 thiE1 (Source: CGSC 6796)
Phenotype: -
Properties: Use of this strain is required for the preparation of single-stranded phage M13 or phasmid DNA to be used in the site-directed mutagenesis method of Kunkel (Kunkel, 1985).
Further information: -
Original reference: Kunkel, Proc. Natl. Acad. Sci. U.S.A. 82 (1985), 488-492 [PMID: 3881765]
Related reference: Warner et al., J. Bacteriol. 145 (1981), 687-695 [PMID: 6109711]
Duncan and Weiss, J. Bacteriol. 151 (1982), 750-755 [PMID: 7047496]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 BW313 (LMBP 1325) is available at BCCM/GeneCorner. It was deposited by Prof. Dr E. Remaut and was published in Kunkel, 1985.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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