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GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli B Glutaminator (LMBP 13330)

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Species:Escherichia coli
Group:B
Strain designation:Glutaminator
Accession number: LMBP 13330
Depositor: Dr T. Mathes1.
1 Experimental Biophysics, Institute of Biology, Humboldt University of Berlin, Berlin, Germany
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Other culture collection numbers: c063
Medium: LB-Lennox
Selection marker: /
Temperature: 28°C
Genotype: F- ompT gal dcm hsdSB (rB- mB-) (DE3) manX::ribM ribC::cat-ribF glnA- asnB- ybaS- gltB- yneH::FRTneoR
Phenotype: Glutamine auxotrophy KanR
Properties: The strain is intended for amino acid specific labelled protein production. As a C41(DE3) derivative, this strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lacUV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase. Protein expression from transforming plasmids containing a T7 promoter-driven expression system is repressed until IPTG induction of T7 RNA polymerase expression occurs. The strain is deficient in the outer membrane protease OmpT which reduces the degradation of heterologous proteins expressed in the strain.
Further information: This strain was created based on E. coli B CpXFΔQ* (LMBP 13326) via site specific chromosomal modification.
Original reference: -
Related reference: Mehlhorn et al., PLoS One. 8 (2013), e79006 [PMID: 24223875] [DOI: 10.1371/journal.pone.0079006]
Mathes et al., J. Mol. Biol. 385 (2009), 1511-1518 [PMID: 19027027] [DOI: 10.1016/j.jmb.2008.11.001]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli B Glutaminator (LMBP 13330) is available at BCCM/GeneCorner. It was deposited by Dr T. Mathes.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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