GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 CSH57 (LMBP 13359)

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Species:Escherichia coli
Strain designation:CSH57
Accession number: LMBP 13359
Depositor: This strain was deposited by Dr F. Van Gijsegem1 and Prof. Dr A. Toussaint1. It was constructed by Prof. Dr J. H. Miller2.
1 Cellular and Molecular Microbiology Unit, Department of Molecular Biology, Université Libre de Bruxelles ULB, Gosselies, Belgium
2 Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California - Los Angeles UCLA, USA
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Other culture collection numbers:CSH 57
Medium: LB-Lennox + streptomycin (100µg/ml)
Selection marker: Streptomycin
Temperature: 37°C
Genotype: F- leu purE trp his argG ilvA met thi mtl gal lacY xyl ara malAts tonA tsx phx supE rpsL
Phenotype: StrepR
Properties: This strain contains auxotrophic mutations evenly spread over the genome. It is a recipient strain for testing the mobilization of complementing alleles from pULB110 and pULB113 by conjugation.
Further information: -
Original reference: Miller, Experiments in molecular genetics, Cold Spring Harbor (N.Y.) : Cold Spring Harbor laboratory, 1972. (1972) [ISSN/ISBN: 0879691069]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 CSH57 (LMBP 13359) is available at BCCM/GeneCorner. It was deposited by Dr F. Van Gijsegem and Prof. Dr A. Toussaint and was published in Miller, 1972.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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