GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 W3110 Osprey (LMBP 13415)

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Species:Escherichia coli
Strain designation:W3110 Osprey
Accession number: LMBP 13415
Depositor: This host strain was deposited by Prof. Dr B. Wren1. It was constructed by Dr E. Kay1.
1 Department of Infection Biology, London School of Hygiene and Tropical Medicine, London, UK
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Other culture collection numbers: -
Medium: LB-Lennox
Selection marker: /
Temperature: 37°C
Genotype: F- λ- IN(rrnD-rrnE)1 rph-1 ΔlpxM ΔwecA-wzzE(gne) ΔwaaL Igs#atpI-gidB(CjpglB) (Source: Kay et al. (2022))
Phenotype: -
Properties: This host strain is a derivative of the Hobby strain and can be used for bioengineering where purified products with reduced endotoxicity are desirable. Enterobacterial common antigen is no longer produced and a UDP N-acetylglucose epimerase has been chromosomally integrated increasing the availability of UDP-GalNAc. The O-antigen ligase has also been removed so that recombinant glycans are not removed from Und-P and attached to surface lipid. The Campylobacter jejuni pglB oligosaccharyltransferase with a 0.1 strength Anderson promoter is integrated into the chromosome of the Hobby strain, between atpI and gidB using CRISPR technology, for bioconjugation.
Further information: This strain is part of a versatile suite of glycoengineering strains for various downstream applications, such as glycoconjugate vaccine production.
Original reference: Kay et al., Microb. Cell Fact. 21 (2022), 66 [PMID: 35449016] [DOI: 10.1186/s12934-022-01792-7]
Restricted use: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.

Refer in your Materials and Methods:

Escherichia coli K12 W3110 Osprey (LMBP 13415) is available at BCCM/GeneCorner. It was deposited by Prof. Dr B. Wren and was published in Kay et al., 2022.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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