Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli B BL21(DE3) (LMBP 1455)
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| Species: | Escherichia coli |
| Group: | B |
| Strain designation: | BL21(DE3) |
| Accession number: | LMBP 1455 |
| Depositor: | Prof. Dr W. Studier1 1 Biology Department, Brookhaven National Laboratory, Upton, USA |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Lennox |
| Selection marker: | / |
| Temperature: | 28°C |
| Genotype: | F- ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) |
| Phenotype: | - |
| Properties: | The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase. Protein expression from transforming plasmids containing a T7 promoter-driven expression system is repressed until IPTG induction of T7 RNA polymerase expression occurs. The strain does not contain the lon protease and is also deficient in the outer membrane protease, OmpT. The lack of two key proteases reduces the degradation of heterologous proteins expressed in the strain. |
| Further information: | There is a complete genome sequence of the BL21(DE3) strain available at Genbank (Accession number: CP001509.3). |
| Original reference: | Studier and Moffatt, J. Mol. Biol. 189 (1986), 113-130 [PMID: 3537305] [DOI: 10.1016/0022-2836(86)90385-2] |
| Related reference: | Daegelen et al., J. Mol. Biol. 394 (2009), 634-643 [PMID: 19765591] [DOI: 10.1016/j.jmb.2009.09.022] Jeong et al., J. Mol. Biol. 394 (2009), 644-652 [PMID: 19786035] [DOI: 10.1016/j.jmb.2009.09.052] Studier et al., J. Mol. Biol. 394 (2009), 653-680 [PMID: 19765592] [DOI: 10.1016/j.jmb.2009.09.021] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli B BL21(DE3) (LMBP 1455) is available at BCCM/GeneCorner. It was deposited by Prof. Dr W. Studier and was published in Studier and Moffatt, 1986. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.