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GeneCorner host details

Last data update: 20 September 2021 09:16 CEST

Host name: Escherichia coli K12 WK6mutS(λ) (LMBP 1466)

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Species:Escherichia coli
Group:K12
Strain designation:WK6mutS(λ)
Accession number: LMBP 1466
Depositor: Prof. Dr E. Remaut1 2
1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Lennox
Selection marker: /
Temperature: 28°C (Note: 37°C is also possible but phage lambda remains more stably integrated when grown at 28°C)
Genotype: F' lacIq Δ(lacZ)M15 proA+B+ traD36/ Δ(lac-proB) galE rpsL mutS::Tn10 λ
Phenotype: StrR TetR
Properties: Useful strain for site-directed mutagenesis; disabled mismatch repair due to knocked-out mutS. The Tn10 insertion renders the strain tetR.
Further information: Not to be used as permanent storage host for plasmids because of the danger of accumulating spontaneous mutations.
Original reference: Zell et al., EMBO J. 6 (1987), 1809-1815 [PMID: 3038536]
Related reference: Stanssens et al., Nucleic Acids Res. 17 (1989), 4441-4454 [PMID: 2501754]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 WK6mutS(λ) (LMBP 1466) is available at BCCM/GeneCorner. It was deposited by Prof. Dr E. Remaut and was published in Zell et al., 1987.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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