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GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 HMS174 (LMBP 1468)

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Species:Escherichia coli
Group:K12
Strain designation:HMS174
Accession number: LMBP 1468
Depositor: This host strain was deposited by Prof. Dr R. Beyaert1 2. It was obtained from Prof. Dr W. Studier 3.
1 VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
3 Biology Department, Brookhaven National Laboratory, Upton, USA
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Other culture collection numbers:CGSC 6576
ATCC 47011
DSM 5932
Medium: LB-Lennox
Selection marker: Rifampicin (50 µg/ml)
Temperature: 37°C
Genotype: F- λ- recA1 IN(rrnD-rrnE)1 rph-1 rpoB331 hsdR19 (Source: CGSC 6576)
Phenotype: RifR
Properties: -
Further information: The Su- recipient for transformations, E. coli K12 HMS174, was derived from the E. coil K12 strains W3110 (thy-) and KLL6-99.

There is a genome sequence of the HMS174 strain available at Genbank (Accession number: NZ_LM993812.1).

The rifampicin resistance has been experimentally verified by BCCM/GeneCorner.
Original reference: Campbell et al., Proc. Natl. Acad. Sci. U.S.A. 75 (1978), 2276-2280 [PMID: 276868] [DOI: 10.1073/pnas.75.5.2276]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 HMS174 (LMBP 1468) is available at BCCM/GeneCorner. It was deposited by Prof. Dr R. Beyaert and was published in Campbell et al., 1978.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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