GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 DH5αF(λ) (LMBP 2846)

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Species:Escherichia coli
Strain designation:DH5αF(λ)
Accession number: LMBP 2846
Depositor: Prof. Dr E. Remaut1 2
1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Lennox
Selection marker: /
Temperature: 28°C (Note: 37°C is also possible but phage lambda remains more stably integrated when grown at 28°C)
Genotype: F+ Δ(argF-lac)169 φ80dlacZ58(M15) ΔphoA8 glnX44(AS) λ+ deoR481 rfbC1 gyrA96 recA1 endA1 thiE1 hsdR17
Phenotype: NalR rK- mK+
Properties: Useful strain for Lacα complementation. This strain does not contain the lacIq gene and therefore repression on the lac promoter, present on high-copy plasmids, is incomplete.
Further information: This strain contains the F factor of NK3 and therefore can be used for infection with M13 or fd phages and derivatives. In addition the strain is lysogenic for wild-type phage λ.

The resistance to nalidixic acid has been experimentally confirmed by BCCM/GeneCorner.
Original reference: -
Related reference: Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8]
Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 DH5αF(λ) (LMBP 2846) is available at BCCM/GeneCorner. It was deposited by Prof. Dr E. Remaut.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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