Last data update: 24 May 2022 11:11 CEST
Host name: Escherichia coli K12 DH5αF(λ) (LMBP 2846)
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|Accession number:||LMBP 2846|
|Depositor:||Prof. Dr E. Remaut1 2
1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Containment level:||This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).|
|Temperature:||28°C (Note: 37°C is also possible but phage lambda remains more stably integrated when grown at 28°C)|
|Genotype:||F+ Δ(argF-lac)169 φ80dlacZ58(M15) ΔphoA8 glnX44(AS) λ+ deoR481 rfbC1 gyrA96 recA1 endA1 thiE1 hsdR17|
|Phenotype:||NalR rK- mK+|
|Properties:||Useful strain for Lacα complementation. This strain does not contain the lacIq gene and therefore repression on the lac promoter, present on high-copy plasmids, is incomplete.|
|Further information:||This strain contains the F factor of NK3 and therefore can be used for infection with M13 or fd phages and derivatives. In addition the strain is lysogenic for wild-type phage λ.
The resistance to nalidixic acid has been experimentally confirmed by BCCM/GeneCorner.
|Related reference:||Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
|Restricted use:||- BCCM MTA|
Refer in your Materials and Methods:
|Escherichia coli K12 DH5αF(λ) (LMBP 2846) is available at BCCM/GeneCorner. It was deposited by Prof. Dr E. Remaut.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.