Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 DH5αF(λ) (LMBP 2846)
New search | Print data sheet |
Species: | Escherichia coli |
Group: | K12 |
Strain designation: | DH5αF(λ) |
Accession number: | LMBP 2846 |
Depositor: | Prof. Dr E. Remaut1 2 1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium 2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
Medium: | LB-Lennox |
Selection marker: | / |
Temperature: | 28°C (Note: 37°C is also possible but phage lambda remains more stably integrated when grown at 28°C) |
Genotype: | F+ Δ(argF-lac)169 φ80dlacZ58(M15) ΔphoA8 glnX44(AS) λ+ deoR481 rfbC1 gyrA96 recA1 endA1 thiE1 hsdR17 |
Phenotype: | NalR rK- mK+ |
Properties: | Useful strain for Lacα complementation. This strain does not contain the lacIq gene and therefore repression on the lac promoter, present on high-copy plasmids, is incomplete. |
Further information: | This strain contains the F factor of NK3 and therefore can be used for infection with M13 or fd phages and derivatives. In addition the strain is lysogenic for wild-type phage λ. The resistance to nalidixic acid has been experimentally confirmed by BCCM/GeneCorner. |
Original reference: | - |
Related reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Restricted use: | - BCCM MTA |
Refer in your Materials and Methods: |
Escherichia coli K12 DH5αF(λ) (LMBP 2846) is available at BCCM/GeneCorner. It was deposited by Prof. Dr E. Remaut. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.