Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 MC1061Rif (LMBP 3319)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | MC1061Rif |
| Accession number: | LMBP 3319 |
| Depositor: | Prof. Dr E. Remaut1 2 1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium 2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Lennox |
| Selection marker: | rifampicin (100 µg/ml) |
| Temperature: | 37°C |
| Genotype: | F- Δ(araA-leu)7697 [araD139]B/r Δ(codB-lacI)3 galK16 galE15(GalS) λ- e14- mcrA0 relA1 rpsL150 spoT1 mcrB1 hsdR2 |
| Phenotype: | RifR StrR rK- mK+ |
| Properties: | Rifampicin resistant MC1061 mutant. Useful host for primary transformation. Transforms very well by the CaCl2 method (10exp7/μg). There is no Type I restriction, incoming DNA receives the E. coli K modification. |
| Further information: | As this strain is deleted for the lacI repressor gene, it is not a suitable host for plasmids carrying the lac promoter or derivatives thereof, such as tac, trc, N25/O2 ... promoters. In the absence of repression, continuous transcription from the lac promoter is likely to result in plasmid instability. The rifampicin and streptomycin resistance has been experimentally verified by BCCM/GeneCorner. |
| Original reference: | - |
| Related reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli K12 MC1061Rif (LMBP 3319) is available at BCCM/GeneCorner. It was deposited by Prof. Dr E. Remaut. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.