Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 SM10(λpir) (LMBP 3889)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | SM10(λpir) |
| Accession number: | LMBP 3889 |
| Depositor: | Dr J. J. Mekalanos1 1 Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, USA |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Lennox |
| Selection marker: | kanamycin (50 µg/ml) |
| Temperature: | 37°C |
| Genotype: | thi thr leu tonA lacY supE recA::RP4-2-TcR::Mu KmR λpir |
| Phenotype: | KanR TetS |
| Properties: | Donor strain (mobilizing strain) carrying the transfer genes of the broad host range IncP type plasmid RP4 integrated in its chromosome. Useful for mobilizing mobRP4 plasmids. |
| Further information: | The presence of λpir allows the replication of a defective R6K suicide plasmid and derivatives thereof. The tetracycline sensitivity as well as the kanamycin resistance have been experimentally verified by BCCM/GeneCorner. |
| Original reference: | - |
| Related reference: | Simon et al., Biotechnology 1 (1983), 784-791 [DOI: 10.1038/nbt1183-784] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli K12 SM10(λpir) (LMBP 3889) is available at BCCM/GeneCorner. It was deposited by Dr J. J. Mekalanos. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.