GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 SM10(λpir) (LMBP 3889)

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Species:Escherichia coli
Strain designation:SM10(λpir)
Accession number: LMBP 3889
Depositor: Dr J. J. Mekalanos1
1 Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, USA
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Lennox
Selection marker: kanamycin (50 µg/ml)
Temperature: 37°C
Genotype: thi thr leu tonA lacY supE recA::RP4-2-TcR::Mu KmR λpir
Phenotype: KanR TetS
Properties: Donor strain (mobilizing strain) carrying the transfer genes of the broad host range IncP type plasmid RP4 integrated in its chromosome. Useful for mobilizing mobRP4 plasmids.
Further information: The presence of λpir allows the replication of a defective R6K suicide plasmid and derivatives thereof.

The tetracycline sensitivity as well as the kanamycin resistance have been experimentally verified by BCCM/GeneCorner.
Original reference: -
Related reference: Simon et al., Biotechnology 1 (1983), 784-791 [DOI: 10.1038/nbt1183-784]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 SM10(λpir) (LMBP 3889) is available at BCCM/GeneCorner. It was deposited by Dr J. J. Mekalanos.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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