Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12xB DB3.1 (LMBP 4098)
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Species: | Escherichia coli |
Group: | K12xB |
Strain designation: | DB3.1 |
Accession number: | LMBP 4098 |
Depositor: | Life Technologies Corporation1 1 Waltham, Massachusetts, USA |
Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
Medium: | LB-Lennox |
Selection marker: | streptomycin (25 µg/ml) |
Temperature: | 37°C |
Genotype: | gyrA462 endA1 Δ(sr1-recA) mcrB mrr hsdS20 glnV44 (=supE44) ara14 galK2 lacY1 proA2 rpsL20 xyl5 leuB6 mtl1 |
Phenotype: | StrR rB- mB- |
Properties: | DB3.1 is a HB101 derivative containing the gyrA462 allele which renders the strain resistant to the toxic effects of the ccdB gene. |
Further information: | Original Invitrogen catalogue number: 11782-018. However, this strain is no longer available at Invitrogen. Alternative: ccdB-resistant One Shot ccdB Survival 2 T1R Competent Cells, of which the ccdB-resistance mechanism is not known; it is based on empirical results and the success or failure of the transformation of the cells is plasmid-dependent (personal communication Invitrogen). |
Original reference: | - |
Related reference: | Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324] |
Restricted use: | - BCCM MTA adapted by Life Technologies Corporation |
Refer in your Materials and Methods: |
Escherichia coli K12xB DB3.1 (LMBP 4098) is available at BCCM/GeneCorner. It was deposited by Life Technologies Corporation. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.