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GeneCorner host details

Last data update: 20 September 2021 09:16 CEST

Host name: Escherichia coli K12xB DB3.1 (LMBP 4098)

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Species:Escherichia coli
Group:K12xB
Strain designation:DB3.1
Accession number: LMBP 4098
Depositor: Life Technologies Corporation1
1 Waltham, Massachusetts, USA
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Lennox
Selection marker: streptomycin (25 µg/ml)
Temperature: 37°C
Genotype: gyrA462 endA1 Δ(sr1-recA) mcrB mrr hsdS20 glnV44 (=supE44) ara14 galK2 lacY1 proA2 rpsL20 xyl5 leuB6 mtl1
Phenotype: StrR rB- mB-
Properties: DB3.1 is a HB101 derivative containing the gyrA462 allele which renders the strain resistant to the toxic effects of the ccdB gene.
Further information: Original Invitrogen catalogue number: 11782-018. However, this strain is no longer available at Invitrogen.

Alternative: ccdB-resistant One Shot ccdB Survival 2 T1R Competent Cells, of which the ccdB-resistance mechanism is not known; it is based on empirical results and the success or failure of the transformation of the cells is plasmid-dependent (personal communication Invitrogen).
Original reference: -
Related reference: Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324]
Restricted use: - BCCM MTA adapted by Life Technologies Corporation

Refer in your Materials and Methods:

Escherichia coli K12xB DB3.1 (LMBP 4098) is available at BCCM/GeneCorner. It was deposited by Life Technologies Corporation.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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