Last data update: 16 June 2021 13:31 CEST
Host name: Escherichia coli K12xB DB3.1 (LMBP 4098)
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|Accession number:||LMBP 4098|
|Depositor:||Life Technologies Corporation1
1 Waltham, Massachusetts, USA
|Containment level:||This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).|
|Selection marker:||streptomycin (25 µg/ml)|
|Genotype:||gyrA462 endA1 Δ(sr1-recA) mcrB mrr hsdS20 glnV44 (=supE44) ara14 galK2 lacY1 proA2 rpsL20 xyl5 leuB6 mtl1|
|Phenotype:||StrR rB- mB-|
|Properties:||DB3.1 is a HB101 derivative containing the gyrA462 allele which renders the strain resistant to the toxic effects of the ccdB gene.|
|Further information:||Original Invitrogen catalogue number: 11782-018. However, this strain is no longer available at Invitrogen.
Alternative: ccdB-resistant One Shot ccdB Survival 2 T1R Competent Cells, of which the ccdB-resistance mechanism is not known; it is based on empirical results and the success or failure of the transformation of the cells is plasmid-dependent (personal communication Invitrogen).
|Related reference:||Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324]
|Restricted use:||- BCCM MTA adapted by Life Technologies Corporation|
Refer in your Materials and Methods:
|Escherichia coli K12xB DB3.1 (LMBP 4098) is available at BCCM/GeneCorner. It was deposited by Life Technologies Corporation.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.