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GeneCorner host details

Last data update: 20 September 2021 09:16 CEST

Host name: Escherichia coli K12 S26 (LMBP 4977)

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Species:Escherichia coli
Group:K12
Strain designation:S26
Accession number: LMBP 4977
Depositor: Prof. Dr E. Remaut1 2
1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
It was obtained from Dr A. Garen3
3 Department of Molecular Biology and Biophysics, Yale University, USA
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Other culture collection numbers:CGSC 4620
Medium: LB-Lennox
Selection marker: /
Temperature: 37°C
Genotype: Hfr garB10 fhuA22 phoA4(Am) ompF627(T2R) fadL701(T2R) relA1 pitA10 spoT1 rrnB-2 mcrB1 creC510 (Source: CGSC 4620)
Phenotype: phosphatase negative
Properties: This strain carries the S26 amber mutation (TAG) in the phosphatase gene. This strain is the parental strain of a series of four isogenic strains differing only in their suppressor activity (see accession numbers LMBP 4977 up to and including LMBP 4980).
Further information: -
Original reference: Garen et al., J. Mol. Biol. 14 (1965), 167-178 [PMID: 5327650]
Related reference: Remaut et al., J. Mol. Biol. 71 (1972), 243-261 [PMID: 4564480]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 S26 (LMBP 4977) is available at BCCM/GeneCorner. It was deposited by Prof. Dr E. Remaut and was published in Garen et al., 1965.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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