Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 S26 (LMBP 4977)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | S26 |
| Accession number: | LMBP 4977 |
| Depositor: | Prof. Dr E. Remaut1 2 1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium 2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium It was obtained from Dr A. Garen3 3 Department of Molecular Biology and Biophysics, Yale University, USA |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Other culture collection numbers: | CGSC 4620 |
| Medium: | LB-Lennox |
| Selection marker: | / |
| Temperature: | 37°C |
| Genotype: | Hfr garB10 fhuA22 phoA4(Am) ompF627(T2R) fadL701(T2R) relA1 pitA10 spoT1 rrnB-2 mcrB1 creC510 (Source: CGSC 4620) |
| Phenotype: | phosphatase negative |
| Properties: | This strain carries the S26 amber mutation (TAG) in the phosphatase gene. This strain is the parental strain of a series of four isogenic strains differing only in their suppressor activity (see accession numbers LMBP 4977 up to and including LMBP 4980). |
| Further information: | - |
| Original reference: | Garen et al., J. Mol. Biol. 14 (1965), 167-178 [PMID: 5327650] |
| Related reference: | Remaut et al., J. Mol. Biol. 71 (1972), 243-261 [PMID: 4564480] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli K12 S26 (LMBP 4977) is available at BCCM/GeneCorner. It was deposited by Prof. Dr E. Remaut and was published in Garen et al., 1965. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.