GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 H12R8a (LMBP 4980)

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Species:Escherichia coli
Strain designation:H12R8a
Accession number: LMBP 4980
Depositor: Prof. Dr E. Remaut1 2
1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
It was obtained from Dr A. Garen3
3 Department of Molecular Biology and Biophysics, Yale University, USA
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Lennox
Selection marker: /
Temperature: 37°C
Genotype: Hfr garB10 fhuA22 phoA5(Am) ompF627(T2R) tyrT34(AS) fadL701(T2R) relA1 pitA10 spoT1 phoT-35 rrnB-2 mcrB1 creC510 (Source: CGSC 2597)
Phenotype: phosphatase positive
Properties: This strain carries the H12 amber mutation (TAG) in the phosphatase gene. This strain carries the supF suppressor (inserts tyrosine at the amber position) and is therefore capable of suppressing the H12 phosphatase nonsense mutation, as well as amber mutations in other genes. However, the efficiency of suppression may vary considerably depending on the sequence context of the amber codon.
Further information: -
Original reference: Garen et al., J. Mol. Biol. 14 (1965), 167-178 [PMID: 5327650]
Related reference: Weigert et al., J. Mol. Biol. 14 (1965), 522-527 [PMID: 4286570]
Remaut et al., J. Mol. Biol. 71 (1972), 243-261 [PMID: 4564480]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 H12R8a (LMBP 4980) is available at BCCM/GeneCorner. It was deposited by Prof. Dr E. Remaut and was published in Garen et al., 1965.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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