GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 JM103 (LMBP 549)

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Species:Escherichia coli
Strain designation:JM103
Accession number: LMBP 549
Depositor: This host strain was deposited by Prof. Dr R. Beyaert1 2. It was obtained from Dr J. Messing 3.
1 VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
3 Waksman Institute, Rutgers, State University of New Jersey, Piscataway, USA
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Other culture collection numbers:ATCC 39403
DSM 2829
NCIMB 12044
Medium: LB-Lennox
Selection marker: streptomcin (25 µg/ml)
Temperature: 37°C
Genotype: F' traD36 proA+ proB+ lacIq delta(lacZ)M15 λ- delta(pro-lac) supE hsdR endA1 sbcB15 thi-1 rpsL (Source: ATCC 39403)
Phenotype: StrR
Properties: -
Further information: Escherichia coli JM103 was derived from JM101 and SK1592.

The streptomycin resistance has been experimentally verified by BCCM/GeneCorner.
Original reference: Messing et al., Nucleic Acids Res. 9 (1981), 309-321 [PMID: 6259625] [DOI: 10.1093/nar/9.2.309]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 JM103 (LMBP 549) is available at BCCM/GeneCorner. It was deposited by Prof. Dr R. Beyaert and was published in Messing et al., 1981.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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