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GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 DH1 (LMBP 623)

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Species:Escherichia coli
Group:K12
Strain designation:DH1
Accession number: LMBP 623
Depositor: This host strain was deposited by Prof. Dr R. Beyaert1 2. It was obtained from Dr D. Hanahan3.
1 VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
3 Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, USA
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Other culture collection numbers:CGSC 6040
ATCC 33849
DSM 4235
NCIMB 12045
Medium: LB-Lennox
Selection marker: Nalidixic acid
Temperature: 37°C
Genotype: F- λ- supE44 relA1 gyrA96 recA1 endA1 thi-1 hsdR17 (Source: ATCC 33849)
Phenotype: NalR rk- mk+
Properties: This plasmid host strain has a relatively high transformation frequency.
Further information: DH1, the parent strain of DH5α, was constructed as follows: thyA was introduced into MM294 by selection with 50 µg trimethoprim/ml. This strain (MMT) was mated with KL16-99 (an Hfr for thyA+ recA- (Low, 1968)) and the cells were plated on minimal glycerol plates, where neither parent will grow. Colonies growing on this medium were restreaked and assayed for UV sensitivity. An UV-sensitive isolate was designated DH2. The gyrA mutation was then introduced by selection for growth on 100 µg nalidixic acid/ml, and this recA deriative of MM294 was designated DH1.

There is a complete genome sequence of the DH1 strain available at Genbank (Accession number: CP001637.1).

The nalidixic acid resistance has been experimentally verified by BCCM/GeneCorner.
Original reference: Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 DH1 (LMBP 623) is available at BCCM/GeneCorner. It was deposited by Prof. Dr R. Beyaert and was published in Hanahan, 1983.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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