Last data update: 20 September 2021 09:16 CEST
Host name: Escherichia coli K12 SG4044 (LMBP 670)
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|Accession number:||LMBP 670|
|Depositor:||Prof. E. Remaut1 2
1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
It was obtained from Dr S. Gottesman3
3 Laboratory of Molecular Biology, NCI, NIH, Bethesda, USA
|Containment level:||This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).|
|Genotype:||lon-100 Δ(gal-pgl)324 lac thi rpsL|
|Phenotype:||StrR Lon- non-mucoid|
|Further information:||The Lon protease plays a major role in the proteolytic machinery of E. coli. Lon mutants are highly pleioptropic and produce mucoid colonies that are difficult to handle. The (gal-pgl) deletion eliminates mucoidy and restores normal colony morphology.
Lon mutants may be useful in stabilizing heterologous proteins expressed in E. coli (see e.g. Remaut et al., 1983).
|Original reference:||Gottesman et al., J. Bacteriol. 148 (1981), 265-273 [PMID: 7026534]
|Related reference:||Remaut et al., Nucleic Acids Res. 11 (1983), 4677-4688 [PMID: 6308555]
Gottesman et al., J. Bacteriol. 162 (1985), 1111-1119 [PMID: 3888955]
|Restricted use:||- BCCM MTA|
Refer in your Materials and Methods:
|Escherichia coli K12 SG4044 (LMBP 670) is available at BCCM/GeneCorner. It was deposited by Prof. E. Remaut and was published in Gottesman et al., 1981.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.