Last data update: 20 September 2021 09:16 CEST
Host name: Escherichia coli K12 SG4121 (LMBP 671)
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|Accession number:||LMBP 671|
|Depositor:||Prof. E. Remaut1 2
1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
It was obtained from Dr S. Gottesman3
3 Laboratory of Molecular Biology, NCI, NIH, Bethesda, USA
|Containment level:||This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).|
|Genotype:||lon-100 Δ(gal-pgl)324 lac thi rpsL recA hsdR|
|Phenotype:||StrR Lon- rK- mK+ non-mucoid|
|Further information:||The Lon protease plays a major role in the proteolytic machinery of E. coli. Lon mutants are highly pleioptropic and produce mucoid colonies that are difficult to handle. The (gal-pgl) deletion eliminates mucoidy and restores normal colony morphology.
Lon mutants may be useful in stabilizing heterologous proteins expressed in E. coli (see e.g. Remaut et al., 1983).
In addition, this strain is K12 restriction negative / modification positive, and RecA deficient.
|Related reference:||Remaut et al., Nucleic Acids Res. 11 (1983), 4677-4688 [PMID: 6308555]
Keidel et al., Eur. J. Biochem. 204 (1992), 1141-1148 [PMID: 1312934]
|Restricted use:||- BCCM MTA|
Refer in your Materials and Methods:
|Escherichia coli K12 SG4121 (LMBP 671) is available at BCCM/GeneCorner. It was deposited by Prof. E. Remaut.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.