Last data update: 20 September 2021 09:16 CEST
Host name: Escherichia coli K12 DP50 (LMBP 8014)
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|Accession number:||LMBP 8014|
|Depositor:||M. Mergeay1 and A. Provoost1
1 SCK.CEN, Mol, Belgium
|Containment level:||This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).|
|Other culture collection numbers:||CGSC 6493|
|Medium:||LB-Lennox + thymine (25 µg/ml) + diaminopimelic acid
|Genotype:||F-, fhuA53, dapD8, lacY1 or Δ(cod-lacI)6, glnX44(AS), Δ(gal-uvrB)47, λ-, gyrA29, ΔthyA57, hsdS3 (Source: CGSC 6493)|
|Properties:||This strain is used for propagation of phage lambda and phage lambda Charon.|
|Further information:||The strain number in the SCK collection is CM0241.
The nalidixic acid resistence could not be experimentally confirmed.
|Original reference:||Leder et al., Science 196 (1977), 175-177 [PMID: 322278]
|Restricted use:||- BCCM MTA|
Refer in your Materials and Methods:
|Escherichia coli K12 DP50 (LMBP 8014) is available at BCCM/GeneCorner. It was deposited by M. Mergeay and A. Provoost and was published in Leder et al., 1977.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.