GeneCorner host details

Last data update: 20 September 2021 09:16 CEST

Host name: Escherichia coli K12 DP50 (LMBP 8014)

Add to cart

New search Print data sheet
Species:Escherichia coli
Strain designation:DP50
Accession number: LMBP 8014
Depositor: M. Mergeay1 and A. Provoost1
1 SCK.CEN, Mol, Belgium
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Other culture collection numbers:CGSC 6493
Medium: LB-Lennox + thymine (25 µg/ml) + diaminopimelic acid
(100 µg/ml)
Selection marker: /
Temperature: 37°C
Genotype: F-, fhuA53, dapD8, lacY1 or Δ(cod-lacI)6, glnX44(AS), Δ(gal-uvrB)47, λ-, gyrA29, ΔthyA57, hsdS3 (Source: CGSC 6493)
Phenotype: NalR
Properties: This strain is used for propagation of phage lambda and phage lambda Charon.
Further information: The strain number in the SCK collection is CM0241.

The nalidixic acid resistence could not be experimentally confirmed.
Original reference: Leder et al., Science 196 (1977), 175-177 [PMID: 322278]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 DP50 (LMBP 8014) is available at BCCM/GeneCorner. It was deposited by M. Mergeay and A. Provoost and was published in Leder et al., 1977.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search