Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12xB HB101 (LMBP 8015)
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| Species: | Escherichia coli |
| Group: | K12xB |
| Strain designation: | HB101 |
| Accession number: | LMBP 8015 |
| Depositor: | Prof. Dr E. Remaut1 2 1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium 2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium It was obtained from B. Allet3 3 Glaxo Institute for Molecular Biology S.A., Plan-les-ouates, Geneva, Switzerland |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Other culture collection numbers: | CGSC 12554 ATCC 33694 DSM 1607 |
| Medium: | LB-Lennox |
| Selection marker: | streptomycin (25 μg/ml) |
| Temperature: | 37°C |
| Genotype: | F- araC14 leuB6(Am) Δ(gpt-proA)62 lacY1 glnX44(AS) galK2(Oc) λ- recA13 rpsL20 xylA5 mtl-1 thiE1 [hsdS20] (Source: CGSC 12554) |
| Phenotype: | StrR rB- mB- |
| Properties: | HB101 is a hybrid E. coli K12xB strain used for plasmid propagation. The strain contains the recA13 mutation which minimizes recombination events and aids in insert stability. Additionally, this strain carries the hsdS20(rB-mB-) restriction-minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 is suitable for many molecular biology applications, including cloning with limited amounts of DNA of vectors that do not require α-complementation for blue/white screening. |
| Further information: | The streptomycin resistance has been experimentally verified bij BCCM/GeneCorner. BCCM/GeneCorner also verified the sensitivity of the strain to ampicillin, chloramphenicol, kanamycin, tetracycline, spectinomycin and zeocin. |
| Original reference: | Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022] |
| Related reference: | Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli K12xB HB101 (LMBP 8015) is available at BCCM/GeneCorner. It was deposited by Prof. Dr E. Remaut and was published in Boyer and Roulland-Dussoix, 1969. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.