Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 BHB2688 (LMBP 8016)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | BHB2688 |
| Accession number: | LMBP 8016 |
| Depositor: | M. Mergeay1 and A. Provoost1 1 SCK.CEN, Mol, Belgium |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Other culture collection numbers: | ATCC 35131 DSM 5451 CGSC 13584 |
| Medium: | LB-Lennox |
| Selection marker: | streptomycin (25 μg/ml) |
| Temperature: | 28°C |
| Genotype: | F- λ[Eam4 b2 red3 imm434 cIts Sam7] recA3 IN(rrnD-rrnE)1 rpsL200 (Source: CGSC 13584) |
| Phenotype: | StrR |
| Properties: | The strain is meant for in vitro packaging of bacteriophage lambda and cosmid DNA and is used for the preparation of freeze-thaw lysates (FTL). |
| Further information: | The strain number in the SCK collection is CM0244. |
| Original reference: | Hohn, Methods Enzymol. 68 (1979), 299-309 [PMID: 161604] [DOI: 10.1016/0076-6879(79)68021-7] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli K12 BHB2688 (LMBP 8016) is available at BCCM/GeneCorner. It was deposited by M. Mergeay and A. Provoost and was published in Hohn, 1979. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.