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GeneCorner host details

Last data update: 20 September 2021 09:16 CEST

Host name: Escherichia coli K12 BHB2688 (LMBP 8016)

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Species:Escherichia coli
Group:K12
Strain designation:BHB2688
Accession number: LMBP 8016
Depositor: M. Mergeay1 and A. Provoost1
1 SCK.CEN, Mol, Belgium
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Other culture collection numbers:ATCC 35131
DSM 5451
CGSC 13584
Medium: LB-Lennox
Selection marker: streptomycin (25 μg/ml)
Temperature: 28°C
Genotype: F- λ[Eam4 b2 red3 imm434 cIts Sam7] recA3 IN(rrnD-rrnE)1 rpsL200 (Source: CGSC 13584)
Phenotype: StrR
Properties: The strain is meant for in vitro packaging of bacteriophage lambda and cosmid DNA and is used for the preparation of freeze-thaw lysates (FTL).
Further information: The strain number in the SCK collection is CM0244.
Original reference: Hohn, Methods Enzymol. 68 (1979), 299-309 [PMID: 161604]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 BHB2688 (LMBP 8016) is available at BCCM/GeneCorner. It was deposited by M. Mergeay and A. Provoost and was published in Hohn, 1979.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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