GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 GM48 (LMBP 877)

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Species:Escherichia coli
Strain designation:GM48
Accession number: LMBP 877
Depositor: This host strain was deposited by Prof. Dr R. Beyaert1 2. It was obtained from Dr M. Marinus 3.
1 VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
3 Department of Pharmacology, University of Massachusetts Medical School, Worcester, USA
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Other culture collection numbers:CGSC 5127
DSM 18040
Medium: LB-Lennox
Selection marker: /
Temperature: 37°C
Genotype: F- λ- thr-1 araC14 leuB6(Am) fhuA31 lacY1 tsx-78 glnX44(AS) galK2(Oc) galT22 dcm-6 dam-3 thiE1 (Source: CGSC 5127)
Phenotype: -
Properties: -
Further information: This strain carries dam-3, a rather unstable allele.

If dam- is required together with the dcm- allele, dam-4 or dam-13::Tn9 would be more recommended. An alternative strain would be Escherichia coli K12 GM2198 (LMBP 4435) which is dcm-6 and dam-13::Tn9.
Original reference: Marinus, Mol. Gen. Genet. 127 (1973), 47-55 [PMID: 4589344] [DOI: 10.1007/BF00267782]
Restricted use: - BCCM MTA
- Acknowledge Dr Martin Marinus as originator of the strain

Refer in your Materials and Methods:

Escherichia coli K12 GM48 (LMBP 877) is available at BCCM/GeneCorner. It was deposited by Prof. Dr R. Beyaert and was published in Marinus, 1973.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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