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GeneCorner host details

Last data update: 20 September 2021 09:16 CEST

Host name: Escherichia coli JK242 (LMBP 917)

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Species:Escherichia coli
Group:K12
Strain designation:JK242
Accession number: LMBP 917
Depositor: Prof. Dr R. Beyaert1 2 via Prof. Dr E. Remaut1 2. It was originally obtained from Dr J. Parker3
1 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
2 VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium
3 Department of Microbiology, Southern Illinois University, Illinois, USA
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Lennox + L-asparagine (100 µg/ml)
Selection marker: streptomycin (25 μg/ml)
Temperature: 37°C
Genotype: asnA31 asnB32 thi-1 relA spoT rpsL125 (source: Parker et al., 1983)
Phenotype: StrR
Properties: The JK242 strain is auxotrophic for asparagine because of the inactivated asnA and asnB gene. It carries also the streptomycin-resistant allele, rpsL, a mutation leading to increased translational accuracy (Parker et al., 1983).
Further information: The streptomycin resistance has been experimentally confirmed by BCCM/GeneCorner.
Original reference: Parker et al., Mol. Gen. Genet. 180 (1980), 275-281 [PMID: 6780757]
Related reference: Felton et al., J. Bacteriol. 142 (1980), 221-228 [PMID: 6102983]
Parker et al., J. Biol. Chem. 258 (1983), 10007-10012 [PMID: 6885754]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli JK242 (LMBP 917) is available at BCCM/GeneCorner. It was deposited by Prof. Dr R. Beyaert via Prof. Dr E. Remaut and was published in Parker et al., 1980.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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