Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli JK242 (LMBP 917)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | JK242 |
| Accession number: | LMBP 917 |
| Depositor: | Prof. Dr R. Beyaert1 2 via Prof. Dr E. Remaut1 2. It was originally obtained from Dr J. Parker3 1 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium 2 VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium 3 Department of Microbiology, Southern Illinois University, Illinois, USA |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Lennox + L-asparagine (100 µg/ml) |
| Selection marker: | streptomycin (25 μg/ml) |
| Temperature: | 37°C |
| Genotype: | asnA31 asnB32 thi-1 relA spoT rpsL125 (source: Parker et al., 1983) |
| Phenotype: | StrR |
| Properties: | The JK242 strain is auxotrophic for asparagine because of the inactivated asnA and asnB gene. It carries also the streptomycin-resistant allele, rpsL, a mutation leading to increased translational accuracy (Parker et al., 1983). |
| Further information: | The streptomycin resistance has been experimentally confirmed by BCCM/GeneCorner. |
| Original reference: | Parker et al., Mol. Gen. Genet. 180 (1980), 275-281 [PMID: 6780757] [DOI: 10.1007/BF00425839] |
| Related reference: | Felton et al., J. Bacteriol. 142 (1980), 221-228 [PMID: 6102983] [DOI: 10.1128/jb.142.1.221-228.1980] Parker et al., J. Biol. Chem. 258 (1983), 10007-10012 [PMID: 6885754] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli JK242 (LMBP 917) is available at BCCM/GeneCorner. It was deposited by Prof. Dr R. Beyaert via Prof. Dr E. Remaut and was published in Parker et al., 1980. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.