Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 MC1061 (LMBP 9560)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | MC1061 |
| Accession number: | LMBP 9560 |
| Depositor: | Prof. Dr E. Remaut1 2 1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium 2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Other culture collection numbers: | CGSC 6649 LMBP 00472 |
| Medium: | LB-Lennox |
| Selection marker: | streptomycin (25 μg/ml) |
| Temperature: | 37°C |
| Genotype: | F- Δ(araA-leu)7697 [araD139]B/r Δ(codB-lacI)3 galK16 galE15(GalS) λ- e14- mcrA0 relA1 rpsL150 spoT1 mcrB1 hsdR2 (Source: CGSC 6649) |
| Phenotype: | StrR rK- mK+ ZeoR |
| Properties: | Useful host for primary transformation. Transforms very well by the CaCl2 method (10exp7/µg). There is no Type I restriction; incoming DNA receives the E. coli K modification. As this strain is deleted for the lacI repressor gene, it is not a suitable host for plasmids carrying the lac promoter or derivatives thereof, such as tac, trc, N25/O2, ... promoters. In the absence of repression, continuous transcription from the lac promoter is likely to result in plasmid instability. The zeocin resistance is most probably related to the presence of a functional recA gene (recA+) (personal communication John Wertz, director E. coli Genetick Stock Center). |
| Further information: | MC1061 <- MC1060 <- MC1000 <- M182 (= M65 x Hfr3000 X74) Following Casadaban and Cohen, J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493], the MC1061 strain is an exconjugant of MC1060. This MC1060 strain is an exconjugant of MC1000, which is an exconjugant of M182. Following Wang et al., J. Bacteriol. 194 (2012), 5856-5863 [PMID: 22923592], the M65 strain and the Hfr3000 X74 strain were crossed to create the M182 strain. M65 <- M32 <- HfrC+ (-> see Durfee et al., J. Bacteriol. 190 (2008), 2597-2606 [PMID: 18245285]) Hfr3000 X74 <- Hfr3000 <- HfrH Thi- (= Hfr Hayes Hfr x W677 F-) (-> see Bachmann BJ. (1996), Derivations and Genotypes of Some Mutant Derivatives of Escherichia coli K-12, p 2460–2488, in Neidhardt et al. (eds), Escherichia coli and Salmonella: Cellular and Molecular Biology, 2nd Edition ASM Press, Washington DC.) Following EcoliWiki the Hfr 3000 X74 strain is an X-ray treated isolate of the Hfr 3000 strain, which is a UV treated isolate of the HfrH Thi- strain (= Hfr Hayes Hfr x W677 F-). |
| Original reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli K12 MC1061 (LMBP 9560) is available at BCCM/GeneCorner. It was deposited by Prof. Dr E. Remaut and was published in Casadaban et al., 1980. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.