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GeneCorner host details

Last data update: 23 September 2021 14:51 CEST

Host name: Escherichia coli K12 MC1061 (LMBP 9560)

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Species:Escherichia coli
Group:K12
Strain designation:MC1061
Accession number: LMBP 9560
Depositor: Prof. Dr E. Remaut1 2
1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Other culture collection numbers:CGSC 6649
LMBP 00472
Medium: LB-Lennox
Selection marker: streptomycin (25 μg/ml)
Temperature: 37°C
Genotype: F- Δ(araA-leu)7697 [araD139]B/r Δ(codB-lacI)3 galK16 galE15(GalS) λ- e14- mcrA0 relA1 rpsL150 spoT1 mcrB1 hsdR2 (Source: CGSC 6649)
Phenotype: StrR rK- mK+ ZeoR
Properties: Useful host for primary transformation. Transforms very well by the CaCl2 method (10exp7/µg). There is no Type I restriction; incoming DNA receives the E. coli K modification. As this strain is deleted for the lacI repressor gene, it is not a suitable host for plasmids carrying the lac promoter or derivatives thereof, such as tac, trc, N25/O2, ... promoters. In the absence of repression, continuous transcription from the lac promoter is likely to result in plasmid instability. The zeocin resistance is most probably related to the presence of a functional recA gene (recA+) (personal communication John Wertz, director E. coli Genetick Stock Center).
Further information: -
Original reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 MC1061 (LMBP 9560) is available at BCCM/GeneCorner. It was deposited by Prof. Dr E. Remaut and was published in Casadaban et al., 1980.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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