Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 IM01B (LMBP 9581)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | IM01B |
| Accession number: | LMBP 9581 |
| Depositor: | Dr I. Monk1 1 Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Lennox |
| Selection marker: | / |
| Temperature: | 37°C |
| Genotype: | mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG Δdcm ΩPhelp-hsdMS (CC1-2) ΩPN25-hsdS (CC1-1) |
| Phenotype: | StrR |
| Properties: | This bacterial host strain was derived from E. coli K12 DH10B by deleting the dcm gene encoding cytosine methylation. Additionally, the hsdMS genes encoding methylase and specificity genes (from Staphylococcus aureus clonal complex 1 - MW2) were introduced onto the chromosome at neutral locations via recombineering. The strain can be transformed efficiently with large plasmids due to deoR (nupG) mutation. Plasmids isolated from this E. coli strain transform S. aureus clonal complex 1 - MW2 at high efficiency. |
| Further information: | - |
| Original reference: | Monk et al., MBio 6 (2015), e00308-15 [PMID: 26015493] [DOI: 10.1128/mBio.00308-15] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli K12 IM01B (LMBP 9581) is available at BCCM/GeneCorner. It was deposited by Dr I. Monk and was published in Monk et al., 2015. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.