GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 IM08B (LMBP 9582)

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Species:Escherichia coli
Strain designation:IM08B
Accession number: LMBP 9582
Depositor: Dr I. Monk1
1 Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Lennox
Selection marker: /
Temperature: 37°C
Genotype: mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG Δdcm ΩPhelp-hsdMS (CC8-2) ΩPN25-hsdS (CC8-1)
Phenotype: StrR
Properties: This bacterial host strain was derived from E. coli K12 DH10B by deleting the dcm gene encoding cytosine methylation. Additionally, the hsdMS genes encoding methylase and specificity genes (from Staphylococcus aureus clonal complex 8 - NRS384) were introduced into the chromosome at neutral locations via recombineering. The strain can be transformed efficiently with large plasmids due to deoR (nupG) mutation. Plasmids isolated from this E. coli strain transform S. aureus clonal complex 8 - NRS384 at high efficiency.
Further information: -
Original reference: Monk et al., MBio 6 (2015), e00308-15 [PMID: 26015493] [DOI: 10.1128/mBio.00308-15]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 IM08B (LMBP 9582) is available at BCCM/GeneCorner. It was deposited by Dr I. Monk and was published in Monk et al., 2015.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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