Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 DC10B (LMBP 9585)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | DC10B |
| Accession number: | LMBP 9585 |
| Depositor: | Dr I. Monk1 1 Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Lennox |
| Selection marker: | / |
| Temperature: | 37°C |
| Genotype: | mcrA ∆(mrr-hsdRMS-mcrBC) ϕ80lacZ∆M15 ∆lacX74 recA1 araD139 ∆(ara-leu)7697 galU galK rpsL endA1 nupG dcm- |
| Phenotype: | StrR |
| Properties: | This bacterial host strain is a DNA cytosine methyltransferase negative mutant of E. coli K12 DH10B (DH10B ∆dcm). So, it is a dam+ but dcm- strain. |
| Further information: | It is a universal Staphylococcal cloning host. |
| Original reference: | Monk et al., MBio 3 (2012), e00277-11 [PMID: 22434850] [DOI: 10.1128/mBio.00277-11] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli K12 DC10B (LMBP 9585) is available at BCCM/GeneCorner. It was deposited by Dr I. Monk and was published in Monk et al., 2012. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.