GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 DC10B (LMBP 9585)

Add to cart

New search Print data sheet
Species:Escherichia coli
Strain designation:DC10B
Accession number: LMBP 9585
Depositor: Dr I. Monk1
1 Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Lennox
Selection marker: /
Temperature: 37°C
Genotype: mcrA ∆(mrr-hsdRMS-mcrBC) ϕ80lacZ∆M15 ∆lacX74 recA1 araD139 ∆(ara-leu)7697 galU galK rpsL endA1 nupG dcm-
Phenotype: StrR
Properties: This bacterial host strain is a DNA cytosine methyltransferase negative mutant of E. coli K12 DH10B (DH10B ∆dcm). So, it is a dam+ but dcm- strain.
Further information: It is a universal Staphylococcal cloning host.
Original reference: Monk et al., MBio 3 (2012), e00277-11 [PMID: 22434850] [DOI: 10.1128/mBio.00277-11]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 DC10B (LMBP 9585) is available at BCCM/GeneCorner. It was deposited by Dr I. Monk and was published in Monk et al., 2012.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search