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GeneCorner host details

Last data update: 20 September 2021 09:16 CEST

Host name: Escherichia coli K12 MG1655 seqA-PAmCherry (LMBP 9778)

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Species:Escherichia coli
Group:K12
Strain designation:MG1655 seqA-PAmCherry
Accession number: LMBP 9778
Depositor: Prof. Dr J. Michiels1. It was constructed by T. Swings1
1 Centre of Microbial and Plant Genetics, Department of Microbial and Molecular Systems, KU Leuven, Leuven, Belgium
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Miller
Selection marker: kanamycin (50 µg/ml)
Temperature: 37°C
Genotype: F- λ- ilvG- rfb-50 rph-1 seqA-PAmCherry:km(R) (Source: http://openwetware.org/wiki/E._coli_genotypes#MG1655 + Mika et al., 2015)
Phenotype: KanR
Properties: This strain can be used to study the subcellular localization of the E. coli DNA binding protein SeqA.
Further information: The native seqA gene was fused at its 3' end to the fluorescent protein gene PAmCherry, separated with a linker (ASPPPGRSR) and followed by a kanamycin resistance marker. The entire cassette was integrated in the chromosome.

PAmCherry is a photoactivatable variant of the red fluorescent protein mCherry. PAmCherry is not fluorescent until it is exposed to 350-400 nm light.
Original reference: Mika et al., Faraday Discuss. 184 (2015), 425-450 [PMID: 26449690] [DOI: 10.1039/c5fd00058k]
Restricted use: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.

Refer in your Materials and Methods:

Escherichia coli K12 MG1655 seqA-PAmCherry (LMBP 9778) is available at BCCM/GeneCorner. It was deposited by Prof. Dr J. Michiels and was published in Mika et al., 2015.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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