Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 MG1655 seqA-PAmCherry (LMBP 9778)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | MG1655 seqA-PAmCherry |
| Accession number: | LMBP 9778 |
| Depositor: | Prof. Dr J. Michiels1. It was constructed by T. Swings1 1 Centre of Microbial and Plant Genetics, Department of Microbial and Molecular Systems, KU Leuven, Leuven, Belgium |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Miller |
| Selection marker: | kanamycin (50 µg/ml) |
| Temperature: | 37°C |
| Genotype: | F- λ- seqA-PAmCherry:km(R) (Source: Guyer et al., 1981 and Mika et al., 2015) |
| Phenotype: | KanR |
| Properties: | This strain can be used to study the subcellular localization of the E. coli DNA binding protein SeqA. |
| Further information: | The native seqA gene was fused at its 3' end to the fluorescent protein gene PAmCherry, separated with a linker (ASPPPGRSR) and followed by a kanamycin resistance marker. The entire cassette was integrated in the chromosome. PAmCherry is a photoactivatable variant of the red fluorescent protein mCherry. PAmCherry is not fluorescent until it is exposed to 350-400 nm light. There is a genome sequence of the parental MG1655 strain available at Genbank (Accession number: U00096.3). |
| Original reference: | Mika et al., Faraday Discuss. 184 (2015), 425-450 [PMID: 26449690] [DOI: 10.1039/c5fd00058k] |
| Related reference: | Guyer et al., Cold Spring Harb. Symp. Quant. Biol. 45 (1981), 135-140 [PMID: 6271456] [DOI: 10.1101/sqb.1981.045.01.022] |
| Restricted use: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
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Refer in your Materials and Methods: |
Escherichia coli K12 MG1655 seqA-PAmCherry (LMBP 9778) is available at BCCM/GeneCorner. It was deposited by Prof. Dr J. Michiels and was published in Mika et al., 2015. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.