Last data update: 24 January 2024 16:39 CET
Plasmid name: BH20 (LMBP 7765)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p7765.gb
(View with Genome Compiler) p7765.txt p7765.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Phage MS2 assembly protein gene (A) Phage MS2 coat protein gene (COAT) RT-PCR positive control DNA Histidine tag (His-tag); N-terminal Thrombin recognition site; N-terminal S-tag; N-terminal Histidine tag (His-tag); C-terminal Escherichia coli lac repressor gene (lacI) |
| Promoter: | Escherichia coli lac repressor promoter (lacI) Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | AU2 |
| Further information: | The plasmid was created by ligating a 178 bp DNA sequence, specific for positive control of RT-PCR, into the HindIII site of the AU2 plasmid. The plasmid is useful for the production of armored RNA. The His-tags, the thrombin recognition site and the S-tag are not in frame with the phage MS2 A and COAT genes. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727566.1. The nucleotide sequence of the pET-30b(+) vector part corresponds with the pET-30b(+) sequence file from Novagen (version 25/5/2002). The nucleotide sequences upstream of the f1 origin and downstream from the T7 promoter were sequenced by GeneCorner. The nucleotide sequence of the pMS27 insert corresponds with the BCCM/GeneCorner sequence analysis results and with the sequence analysis results from the depositor. However, the 'C' nucleotide at position 291 of the COAT protein cds was originally a 'T' in the sequence file of pMS27 (LMBP 5349). This nucleotide change concerns a silent mutation. |
| EMBL Accession number: | LT727566.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 10/09/2012 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI, HincII, HindIII/KpnI, PvuII and XbaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr V. Jestin(1). (1) VIPAC unit, National reference laboratory on avian influenza & Newcastle disease, Ploufragan, France |
| Plasmid reference: | - |
| Related plasmid reference: | Yu et al., J. Clin. Microbiol. 46 (2008), 837-841 [PMID: 18160451] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli B BL21(DE3) |
| Host reference: | Studier and Moffatt, J. Mol. Biol. 189 (1986), 113-130 [PMID: 3537305] [DOI: 10.1016/0022-2836(86)90385-2] |
| Related host reference: | Daegelen et al., J. Mol. Biol. 394 (2009), 634-643 [PMID: 19765591] [DOI: 10.1016/j.jmb.2009.09.022] Jeong et al., J. Mol. Biol. 394 (2009), 644-652 [PMID: 19786035] [DOI: 10.1016/j.jmb.2009.09.052] Studier et al., J. Mol. Biol. 394 (2009), 653-680 [PMID: 19765592] [DOI: 10.1016/j.jmb.2009.09.021] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | Take a low-copy-plasmid yield into account while choosing a plasmid DNA isolation procedure. |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
BH20 (LMBP 7765) is available at BCCM/GeneCorner. This plasmid was deposited by Dr V. Jestin. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.