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GeneCorner plasmid details

Last data update: 03 December 2020 04:26 CET

Plasmid name: Col1a1 5'pEntry (LMBP 9521)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9521.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Escherichia coli lac operon promoter
Human cytomegalovirus immediate early promoter (CMV-IE); minimal promoter with tet operator (CMVT)
Ribosome
binding site:
-
Terminator: -
Selection marker: Neomycin (neo; kanamycin (kan))
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pUC57
Further information: The plasmid is a multisite Gateway entry vector, designed as a 5' entry clone (with attL4-attR1 sites).
The plasmid contains:
- a tetracycline responsive element (TRE), consisting of the minimal human CMV promoter, combined with the tet operator.
- two adjacent copies of the chicken β-globin 5' DNase I hypersensitive site 4 (5'HS4) insulator core sequence. Insulators are DNA sequences which possess the ability to protect expressing genes from inappropriate signals emanating from their surrounding environment by acting as barriers that prevent the advance of nearby condensed chromatin that may otherwise silence expression.
This plasmid is meant for targeting the mouse Col1a1 locus to make inducible, conditional transgenic mice. It should be used in combination with:
- a middle entry clone with attL1-attL2 sites and containing the gene of interest
- a 3' entry clone with attR2-attL3 sites
EMBL Accession number: Y14837, view at EMBL, GenBank, DDBJ
Latest sequence update: 27/02/2015
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: The plasmid was deposited by Dr T. Hochepied(1)(2) and Prof. Dr C. Libert(1)(2).
(1) Inflammation Research Center, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + kanamycin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

Col1a1 5'pEntry (LMBP 9521) is available at BCCM/GeneCorner. The plasmid was deposited by Dr T. Hochepied and Prof. Dr C. Libert.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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