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GeneCorner plasmid details

Last data update: 19 October 2020 04:30 CEST

Plasmid name: FlacproMu18ACm (LMBP 7989)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli lac Z gene (lacZ)
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Chloramphenicol (cam)
Methyl-β-D-galactopyranoside (Tra+)
Streptomycin (Sm)
PRO; auxotrophic
Replicon: -
Host range: Escherichia coli
Parental clone: -
Further information: The plasmid was constructed in vivo based on natural plasmids. It is meant for in vivo cloning.
This plasmid belongs to the IncF incompatibility family.
The plasmid posesses chromosome mobilisation ability (Cma+).
The plasmid contains the MiniMu transposon which is highly efficient in Enterobacteria but can be used in other bacteria as well.
The plasmid contains prophages Mucts62 and Mu18ACm.
EMBL Accession number: -
Latest sequence update: 18/03/2013
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by M. Mergeay(1) and A. Provoost(1).
(1) SCK.CEN, Mol, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 CSH41
Host reference: -
Cultivation medium: LB-Lennox + chloramphenicol (34 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: SCK CM0188

Refer in your Materials and Methods:

FlacproMu18ACm (LMBP 7989) is available at BCCM/GeneCorner. This plasmid was deposited by M. Mergeay and A. Provoost.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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