Last data update: 24 January 2024 16:39 CET
Plasmid name: FlacproMu18ACm (LMBP 7989)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli lac Z gene (lacZ) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Chloramphenicol (cam) Methyl-β-D-galactopyranoside (Tra+) Streptomycin (Sm) PRO; auxotrophic |
| Replicon: | - |
| Host range: | Escherichia coli |
| Parental clone: | - |
| Further information: | The plasmid was constructed in vivo based on natural plasmids. It is meant for in vivo cloning. This plasmid belongs to the IncF incompatibility family. The plasmid posesses chromosome mobilisation ability (Cma+). The plasmid contains the MiniMu transposon which is highly efficient in Enterobacteria but can be used in other bacteria as well. The plasmid contains prophages Mucts62 and Mu18ACm. |
| EMBL Accession number: | - |
| Latest sequence update: | 18/03/2013 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by M. Mergeay(1) and A. Provoost(1). (1) SCK.CEN, Mol, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 CSH41 |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + chloramphenicol (34 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | SCK CM0188 |
Refer in your Materials and Methods: |
FlacproMu18ACm (LMBP 7989) is available at BCCM/GeneCorner. This plasmid was deposited by M. Mergeay and A. Provoost. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.