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GeneCorner plasmid details

Last data update: 27 October 2020 04:27 CET

Plasmid name: LNXCO3 (LMBP 4228)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p4228.gb (View with Genome Compiler)
p4228.txt
p4228.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer; with 3 lac operators downstream from the transcription start site
Moloney mouse sarcoma virus long terminal repeat (5' MMSV-LTR)
Ribosome
binding site:
-
Terminator: Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA)
Moloney mouse leukemia virus long terminal repeat (3' MMLV-LTR)
Selection marker: Ampicillin (amp)
Neomycin (neo; G418)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; preferably recombination-deficient strains
Mammalian cells
Parental clone: pLNCX; pOPI3CAT
Further information: LNXCO3 is a lac repressor-regulated retroviral expression vector.
It contains the CO3 unit consisting of the human CMV-IE promoter and enhancer, three lac operators inserted in the SV40 small t-antigen intron sequence downstream from the transcription start site and the HSV-TK polyA signal.
The orientation of the hCMV-IE promoter is antisense relative to the 3' MMLV-LTR.
18/11/2010
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727330.1.
The nucleotide sequence of the parental pLNCX vector corresponds with the EMBL Nucleotide Sequence Database accession number M28247.1.
Other name of the plasmid is LNXCO3.BN.
EMBL Accession number: M28247.1, view at EMBL, GenBank, DDBJ
LT727330.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 03/10/2000
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI, NcoI/SpeI, NdeI, NotI, PvuI/SspI and XbaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr I. Roninson(1). It was constructed by B. Chang(1).
(1) Department of Molecular Genetics, University of Illinois at Chicago, USA
Plasmid reference: Chang et al., Gene 183 (1996), 137-142 [PMID: 8996098]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1 in E. coli; L2 in mammalian cells
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

LNXCO3 (LMBP 4228) is available at BCCM/GeneCorner. This plasmid was deposited by Dr I. Roninson and was published in Chang et al., 1996.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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