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GeneCorner plasmid details

Last data update: 30 October 2020 04:11 CET

Plasmid name: LNXCO4 (LMBP 4229)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p4229.gb (View with Genome Compiler)
p4229.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Moloney mouse leukemia virus long terminal repeat (5' MMLV-LTR)
Ribosome
binding site:
-
Terminator: Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA)
Moloney mouse leukemia virus long terminal repeat (3' MMLV-LTR)
Selection marker: Ampicillin (amp)
Neomycin (neo; G418)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; preferably recombination-deficient strains
Mammalian cells
Parental clone: pLNCX; pOPI3CAT
Further information: LNXCO4 is a lac repressor-regulated retroviral expression vector.
It contains the CO4 unit, consisting of the human cytomegalovirus immediate early promoter (CMV-IE), with three lac operators inserted downstream from and one lac operator upstream of the transcription start in the SV40 intron sequence, and the Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA).
Unique BglII, HpaI, SgfI and NotI sites are available for cloning.
The CMV promoter is placed antisense relative to the Moloney mouse leukemia virus long terminal repeat (MMLV-LTR).
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727331.1.
EMBL Accession number: LT727331.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 03/10/2000
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI, NcoI/SpeI, NdeI, NotI, PvuI/SspI, SpeI/XbaI and XbaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr I. Roninson(1) and constructed by B. Chang.
(1) Department of Molecular Genetics, University of Illinois at Chicago, USA
Plasmid reference: Chang et al., Gene 183 (1996), 137-142 [PMID: 8996098]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1 in E. coli; L2 in mammalian cells
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

LNXCO4 (LMBP 4229) is available at BCCM/GeneCorner. This plasmid was deposited by Dr I. Roninson and constructed by B. Chang.
Department of Molecular Genetics, University of Illinois at Chicago, USA and was published in Chang et al., 1996.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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