Last data update: 24 January 2024 16:39 CET
Plasmid name: ROSA-sponge125 (LMBP 9058)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9058.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Aequorea macrodactyla green fluorescent protein DNA (GFP); enhanced yellow-green fluorescent variant (EYFP), modified sequence |
| Promoter: | Mouse phosphoglycerate kinase 1 promoter (PGK1) Phage T3 promoter Phage T7 gene 10 promoter (T7g10) Escherichia coli lac operon promoter Escherichia coli lac operon promoter; mutant (lacUV5) |
| Ribosome binding site: |
Internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) polyprotein gene |
| Terminator: | Mouse phosphoglycerate kinase 1 polyadenylation signal (PGK1 polyA) Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Chloramphenicol (cam) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pROSA26-DV1; pENT-sponge125 |
| Further information: | The plasmid was created via LR reaction between pROSA26-DV1 and pENT-sponge125. |
| EMBL Accession number: | - |
| Latest sequence update: | 31/03/2014 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr M. Farhang(1) (2) and Prof. Dr G. Van Loo(1) (2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Nyabi et al., Nucleic Acids Res. 37 (2009), e55 [PMID: 19279185] [DOI: 10.1093/nar/gkp112] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
ROSA-sponge125 (LMBP 9058) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Farhang and Prof. Dr G. Van Loo . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.