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GeneCorner plasmid details

Last data update: 24 January 2024 16:39 CET

Plasmid name: colE1-kam (LMBP 7998)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Neomycin (neo; kanamycin (kan))
Replicon: -
Host range: Escherichia coli
Parental clone: -
Further information: The plasmid was constructed in vivo based on natural plasmids.
The plasmid is an ancestor of the pBR plasmid family.
EMBL Accession number: -
Latest sequence update: 18/03/2013
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by M. Mergeay(1) and A. Provoost(1).
(1) SCK.CEN, Mol, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 W3110
Host reference: -
Related host reference: Bachmann, Bacteriol. Rev. 36 (1972), 525-557 [PMID: 4568763] [DOI: 10.1128/br.36.4.525-557.1972]
Cultivation medium: LB-Lennox + kanamycin (50 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: SCK CM0206

Refer in your Materials and Methods:

colE1-kam (LMBP 7998) is available at BCCM/GeneCorner. This plasmid was deposited by M. Mergeay and A. Provoost.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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