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GeneCorner plasmid details

Last data update: 31 October 2020 04:21 CET

Plasmid name: gGFP (LMBP 8052)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p8052.gb (View with Genome Compiler)
p8052.txt
p8052.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Synthetic green fluorescent protein DNA (SGFP)
Promoter: Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase promoter (gpdA)
Ribosome
binding site:
-
Terminator: Aspergillus nidulans tryptophan C terminator (trpC)
Selection marker: Ampicillin (amp)
Hygromycin (hyg)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Ascomycete fungi
Parental clone: pAN52-1; pHSP70-SG; pAN701
Further information: The plasmid was constructed by cloning the BglII/NcoI fragment from pAN52-1, containing the gdpA promoter, into the BglII/NcoI opened pHSP70-SG vector, upstream of the SGFP coding sequence. The gpdA-hyg-trpC cassette from pAN701 was excised as a BglII/BamHI fragment and cloned into the BglII site of the newly created vector.
The nucleotide sequence of the gdpA promoter corresponds with the EMBL Nucleotide Sequence Database accession number M33539.1.
The nucleotide sequence of the trpC terminator corresponds with the EMBL Nucleotide Sequence Database accession number X02390.1.
Since the nucleotide sequence of the gGFP vector could not be completely traced back, there is uncertainty as to the cutting frequency of the restriction enzymes, except for the sites that were analysed in the authenticity test.
EMBL Accession number: M33539.1, view at EMBL, GenBank, DDBJ
X02390.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 28/05/2013
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, BglII, EcoRI, PvuI, PvuII and SalI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr K. McCluskey(1).
(1) FGSC, University of Missouri, Kansas City, USA
Plasmid reference: Maor et al., Mycol. Res. 102 (1998), 491-496
Related plasmid reference: Kaufman et al., Microbiology 150 (2004), 2785-2790 [PMID: 15289574]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

gGFP (LMBP 8052) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K. McCluskey and was published in Maor et al., 1998.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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