Last data update: 31 October 2020 04:21 CET
Plasmid name: gGFP (LMBP 8052)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Cloned DNA:||Synthetic green fluorescent protein DNA (SGFP)
|Promoter:||Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase promoter (gpdA)|
|Terminator:||Aspergillus nidulans tryptophan C terminator (trpC)|
|Selection marker:||Ampicillin (amp)
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli
|Parental clone:||pAN52-1; pHSP70-SG; pAN701|
|Further information:||The plasmid was constructed by cloning the BglII/NcoI fragment from pAN52-1, containing the gdpA promoter, into the BglII/NcoI opened pHSP70-SG vector, upstream of the SGFP coding sequence. The gpdA-hyg-trpC cassette from pAN701 was excised as a BglII/BamHI fragment and cloned into the BglII site of the newly created vector.
The nucleotide sequence of the gdpA promoter corresponds with the EMBL Nucleotide Sequence Database accession number M33539.1.
The nucleotide sequence of the trpC terminator corresponds with the EMBL Nucleotide Sequence Database accession number X02390.1.
Since the nucleotide sequence of the gGFP vector could not be completely traced back, there is uncertainty as to the cutting frequency of the restriction enzymes, except for the sites that were analysed in the authenticity test.
|EMBL Accession number:||M33539.1, view at EMBL, GenBank, DDBJ
X02390.1, view at EMBL, GenBank, DDBJ
|Latest sequence update:||28/05/2013|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BamHI, BglII, EcoRI, PvuI, PvuII and SalI.|
|History of deposit:||This plasmid was deposited by Prof. Dr K. McCluskey(1).
(1) FGSC, University of Missouri, Kansas City, USA
|Plasmid reference:||Maor et al., Mycol. Res. 102 (1998), 491-496
|Related plasmid reference:||Kaufman et al., Microbiology 150 (2004), 2785-2790 [PMID: 15289574]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5α|
|Host reference:||Focus 8 (1986), 9
|Related host reference:||Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|gGFP (LMBP 8052) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K. McCluskey and was published in Maor et al., 1998.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.