Last data update: 24 January 2024 16:39 CET
Plasmid name: gGFP (LMBP 8052)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
p8052.gb
(View with Genome Compiler) p8052.txt p8052.pdf |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Synthetic green fluorescent protein DNA (SGFP) |
Promoter: | Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase promoter (gpdA) |
Ribosome binding site: |
- |
Terminator: | Aspergillus nidulans tryptophan C terminator (trpC) |
Selection marker: | Ampicillin (amp) Hygromycin (hyg) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli Ascomycete fungi |
Parental clone: | pAN52-1; pHSP70-SG; pAN701 |
Further information: | The plasmid was constructed by cloning the BglII/NcoI fragment from pAN52-1, containing the gdpA promoter, into the BglII/NcoI opened pHSP70-SG vector, upstream of the SGFP coding sequence. The gpdA-hyg-trpC cassette from pAN701 was excised as a BglII/BamHI fragment and cloned into the BglII site of the newly created vector. The nucleotide sequence of the gdpA promoter corresponds with the EMBL Nucleotide Sequence Database accession number M33539.1. The nucleotide sequence of the trpC terminator corresponds with the EMBL Nucleotide Sequence Database accession number X02390.1. Since the nucleotide sequence of the gGFP vector could not be completely traced back, there is uncertainty as to the cutting frequency of the restriction enzymes, except for the sites that were analysed in the authenticity test. |
EMBL Accession number: | M33539.1, view at EMBL, GenBank, DDBJ X02390.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 28/05/2013 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, BglII, EcoRI, PvuI, PvuII and SalI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr K. McCluskey(1). (1) FGSC, University of Missouri, Kansas City, USA |
Plasmid reference: | Maor et al., Mycol. Res. 102 (1998), 491-496 |
Related plasmid reference: | Kaufman et al., Microbiology 150 (2004), 2785-2790 [PMID: 15289574] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
gGFP (LMBP 8052) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K. McCluskey and was published in Maor et al., 1998. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.