GeneCorner plasmid details

Last data update: 20 September 2021 09:16 CEST

Plasmid name: p220.2 (LMBP 1966)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: (View with Genome Compiler)
analysis results


Cloned DNA: Epstein-Barr virus nuclear antigen 1 gene (EBNA1, EBNA-1, GeneID 3783709)
Promoter: Herpes simplex virus (HSV) thymidine kinase promoter (TK)
binding site:
Terminator: Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA)
Selection marker: Ampicillin (amp)
Hygromycin (hyg)
Replicon: Escherichia coli plasmid pMB1 origin
Epstein-Barr virus origin of replication (oriP)
Host range: Escherichia coli
Mammalian cells
Parental clone: p201
Further information: This plasmid is an EBNA1 expression plasmid.
EBNA1 is a trans-acting factor which allows episomal maintenance of oriP-bearing plasmids. It is the only virally encoded protein necessary for plasmid maintenance. The protein contains DNA-linking domains which contribute to the activation of transcription and replication, a DNA-binding and dimerization domain, a chromosome binding domain and a gly-ala repeat. The central domain of EBNA1 is entirely composed of a repetitive array of glycine and alanine residues. The majority of this domain can be deleted without affecting the EBNA1 function.
The bacterial gene for hygromycin B resistance is expressed under control of the promoter and polyadenylation signals of the Herpes simplex thymidine kinase gene.
Addition of hygromycin to the cell line cultivation medium prevents the decrease of p220.2 copies.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727609.1.
EMBL Accession number: LT727609.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 16/06/1992
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AvaI, EcoRI and EcoRV.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr B. Sugden(1).
(1) McArdle Laboratory for Cancer Research, Wisconsin, USA
Plasmid reference: Yates et al., Nature 313 (1985), 812-815 [PMID: 2983224]
Related plasmid reference: Van Craenenbroeck et al., Gene 253 (2000), 293-301 [PMID: 10940567]
Van Craenenbroeck et al., Eur. J. Biochem. 267 (2000), 5665-5678 [PMID: 10971576]
Sugden et al., Mol. Cell. Biol. 5 (1985), 410-413 [PMID: 2983194]
McKnight, Nucleic Acids Res. 8 (1980), 5949-5964 [PMID: 6258156]
Gritz et al., Gene 25 (1983), 179-188 [PMID: 6319235]
Baer et al., Nature 310 (1984), 207-211 [PMID: 6087149]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

p220.2 (LMBP 1966) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr B. Sugden and was published in Yates et al., 1985.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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