Last data update: 24 January 2024 16:39 CET
Plasmid name: p220.2MxCAT or.2 (LMBP 4523)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Herpes simplex virus (HSV) thymidine kinase promoter (TK) |
| Ribosome binding site: |
- |
| Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) |
| Selection marker: | Ampicillin (amp) Hygromycin (hyg) Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin Epstein-Barr virus origin of replication (oriP) |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | p220.2 |
| Further information: | - |
| EMBL Accession number: | - |
| Latest sequence update: | 25/04/2005 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed in the research unit of Em. Prof. Dr G. Haegeman(2) by Dr K. Van Craenenbroeck(2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Van Craenenbroeck et al., Nucleic Acids Res., (submitted) |
| Related plasmid reference: | Van Craenenbroeck et al., Gene 253 (2000), 293-301 [PMID: 10940567] Van Craenenbroeck et al., Eur. J. Biochem. 267 (2000), 5665-5678 [PMID: 10971576] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
p220.2MxCAT or.2 (LMBP 4523) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert and was published in Van Craenenbroeck et al., . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.