GeneCorner plasmid details

Last data update: 23 July 2021 09:43 CEST

Plasmid name: p35S:16xBoxB-Gateway-BASTA (LMBP 9017)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence:
analysis results


Cloned DNA: B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene)
Phage λ promoter-proximal element (nut); RNA stem loop (boxB, box-B); N-terminal
Promoter: Cauliflower mosaic virus (CaMV) 35S promoter
Agrobacterium tumefaciens Ti-plasmid mannopine synthase promoter (mas)
Escherichia coli lac operon promoter; mutant (lacUV5)
binding site:
Terminator: Agrobacterium tumefaciens Ti-plasmid mannopine synthase terminator (mas)
Agrobacterium tumefaciens Ti-plasmid octopine synthase terminator (ocs)
Selection marker: Phosphinotricin (basta)
Chloramphenicol (cam)
Kanamycin (kan)
Replicon: Escherichia coli plasmid pMB1 origin
Pseudomonas plasmid pVS1 origin
Host range: Escherichia coli; use a ccdB-resistant strain for propagation
Plant cells
Gram-negative bacterial strains
Further information: This Gateway destination vector is part of a two-component system for in vivo visualisation of RNA in plant cells.
The vector is meant to introduce target RNA, fused to 16 N-terminal boxB loops, which are recognised by the λN22 protein present in one of the corresponding fluorescence markers vectors (LMBP 9019 up to and including 9025).
The λN22/box-B system is well suited for low-background and high-throughput studies.
Other names of the plasmid are pSCJ232 and p35S:16xBoxB-Gateway BASTA.
EMBL Accession number: -
Latest sequence update: 23/05/2014
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: KpnI, NotI and XhoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr U. Hammes(1).
(1) Department of Cell Biology and Plant Biochemistry, University of Regensburg, Regensburg, Germany
Plasmid reference: Schönberger et al., Plant J. 71 (2012), 173-181 [PMID: 22268772]
Related plasmid reference: Daigle et al., Nat. Methods 4 (2007), 633-636 [PMID: 17603490]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB DB3.1
Host reference: -
Related host reference: Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324]
Cultivation medium: LB-Lennox + chloramphenicol (34 μg/ml) + kanamycin (50 μg/ml)*
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with kanamycin.
Other culture collection numbers: -

Refer in your Materials and Methods:

p35S:16xBoxB-Gateway-BASTA (LMBP 9017) is available at BCCM/GeneCorner. This plasmid was deposited by Dr U. Hammes and was published in Schönberger et al., 2012.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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