Last data update: 12 April 2021 09:56 CEST
Plasmid name: p35S:16xBoxB-Gateway-BASTA (LMBP 9017)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene)|
Phage λ promoter-proximal element (nut); RNA stem loop (boxB, box-B); N-terminal
|Promoter:||Cauliflower mosaic virus (CaMV) 35S promoter
Agrobacterium tumefaciens Ti-plasmid mannopine synthase promoter (mas)
Escherichia coli lac operon promoter; mutant (lacUV5)
|Terminator:||Agrobacterium tumefaciens Ti-plasmid mannopine synthase terminator (mas)
Agrobacterium tumefaciens Ti-plasmid octopine synthase terminator (ocs)
|Selection marker:||Phosphinotricin (basta)
|Replicon:||Escherichia coli plasmid pMB1 origin
Pseudomonas plasmid pVS1 origin
|Host range:||Escherichia coli; use a ccdB-resistant strain for propagation
Gram-negative bacterial strains
|Further information:||This Gateway destination vector is part of a two-component system for in vivo visualisation of RNA in plant cells.
The vector is meant to introduce target RNA, fused to 16 N-terminal boxB loops, which are recognised by the λN22 protein present in one of the corresponding fluorescence markers vectors (LMBP 9019 up to and including 9025).
The λN22/box-B system is well suited for low-background and high-throughput studies.
Other names of the plasmid are pSCJ232 and p35S:16xBoxB-Gateway BASTA.
|EMBL Accession number:||-|
|Latest sequence update:||23/05/2014|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: KpnI, NotI and XhoI.|
|History of deposit:||This plasmid was deposited by Dr U. Hammes(1).
(1) Department of Cell Biology and Plant Biochemistry, University of Regensburg, Regensburg, Germany
|Plasmid reference:||Schönberger et al., Plant J. 71 (2012), 173-181 [PMID: 22268772]
|Related plasmid reference:||Daigle et al., Nat. Methods 4 (2007), 633-636 [PMID: 17603490]
|Restricted distribution:||- BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12xB DB3.1|
|Related host reference:||Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324]
|Cultivation medium:||LB-Lennox + chloramphenicol (34 μg/ml) + kanamycin (50 μg/ml)*|
|Cultivation remark:||*: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with kanamycin.|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|p35S:16xBoxB-Gateway-BASTA (LMBP 9017) is available at BCCM/GeneCorner. This plasmid was deposited by Dr U. Hammes and was published in Schönberger et al., 2012.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.