Last data update: 24 January 2024 16:39 CET
Plasmid name: p35S:6xMS2-Gateway-BASTA (LMBP 9026)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p9026.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene) Phage MS2 coat protein binding stem loop (MS2); N-terminal |
Promoter: | Cauliflower mosaic virus (CaMV) 35S promoter Agrobacterium tumefaciens Ti-plasmid mannopine synthase promoter (mas) Escherichia coli lac operon promoter; mutant (lacUV5) |
Ribosome binding site: |
- |
Terminator: | Agrobacterium tumefaciens Ti-plasmid mannopine synthase terminator (mas) Agrobacterium tumefaciens Ti-plasmid octopine synthase terminator (ocs) |
Selection marker: | Phosphinotricin (basta) Chloramphenicol (cam) Kanamycin (kan) |
Replicon: | Escherichia coli plasmid pMB1 origin Pseudomonas plasmid pVS1 origin |
Host range: | Escherichia coli; use a ccdB-resistant strain for propagation Plant cells Gram-negative bacterial strains |
Further information: | This Gateway destination vector is part of a two-component system for in vivo visualisation of RNA in plant cells. The vector is meant to introduce target RNA, fused to 6 N-terminal MS2 loops, which are recognised by the MS2-CP protein present in one of the corresponding fluorescence markers vectors (LMBP 9028 up to and including 9035). Other names of the plasmid are pSCJ216 and p35S:6xMS2-Gateway BASTA. |
EMBL Accession number: | - |
Latest sequence update: | 23/05/2014 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRV, KpnI, NotI and XhoI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr U. Hammes(1). (1) Department of Cell Biology and Plant Biochemistry, University of Regensburg, Regensburg, Germany |
Plasmid reference: | Schönberger et al., Plant J. 71 (2012), 173-181 [PMID: 22268772] |
Related plasmid reference: | Daigle et al., Nat. Methods 4 (2007), 633-636 [PMID: 17603490] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12xB DB3.1 |
Host reference: | - |
Related host reference: | Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324] |
Cultivation medium: | LB-Lennox + chloramphenicol (34 μg/ml) + kanamycin (50 μg/ml)* |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with kanamycin. |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
p35S:6xMS2-Gateway-BASTA (LMBP 9026) is available at BCCM/GeneCorner. This plasmid was deposited by Dr U. Hammes and was published in Schönberger et al., 2012. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.