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GeneCorner plasmid details

Last data update: 20 October 2020 04:14 CEST

Plasmid name: p3SR2 (LMBP 2363)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p2363.gb (View with Genome Compiler)
p2363.txt
p2363.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Aspergillus nidulans acetamidase gene (amdS)
Promoter: -
Ribosome
binding site:
-
Terminator: Aspergillus nidulans acetamidase terminator (amdS)
Selection marker: Ampicillin (amp)
Acetamidase (amdS)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Fungi; integrative
Parental clone: pBR322
Further information: The plasmid was constructed by cloning a 5 kb EcoRI-SalI fragment from the Charon 4A gene bank, containing the acetamidase gene from A. nidulans, into the unique EcoRI and unique SalI site of pBR322.
The nucleotide sequence of the amdS gene corresponds with the EMBL Nucleotide Sequence Database accession number M16371.1.
Since the nucleotide sequence at the 5' and 3' end of the insert could not be completely traced back, there is uncertainty as to the cutting frequency of the restriction enzymes, except for the sites that were analysed in the authenticity test.
EMBL Accession number: M16371.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 24/10/1991
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AvaI, BsaAI, EcoRI, EcoRI/SalI, EcoRI/XcmI and XcmI.

The results 1) EcoRI, SalI and XcmI are really unique sites; 2) The plasmid length seems to be approx. 9000 bp. The approximately 200 extra nucleotides are located between the EcoRI site and the Xcm site, most probably in the unknown sequence region; 3) The orientation of the acetamidase insert could be confirmed; 4) An extra AvaI site and an extra BsaAI site have been found in the acetamidase terminator region.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr M.J. Hynes(1).
(1) University of Melbourne, Australia.
Plasmid reference: Hynes et al., Mol. Cell. Biol. 3 (1983), 1430-1439 [PMID: 6353203]
Related plasmid reference: Corrick et al., Gene 53 (1987), 63-71 [PMID: 3036667]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

p3SR2 (LMBP 2363) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M.J. Hynes and was published in Hynes et al., 1983.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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