Last data update: 29 November 2020 04:28 CET
Plasmid name: p415-CFP-N for C (LMBP 9735)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Cloned DNA:||Aequorea victoria green fluorescent protein DNA (GFP); enhanced cyan fluorescent variant (Cerulean), N-terminal fragment, C-terminal
|Promoter:||Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1)
Phage T3 promoter
Phage T7 gene 10 promoter (T7g10); synthetic sequence
Escherichia coli lac operon promoter
|Terminator:||Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)|
|Selection marker:||Ampicillin (amp)
|Replicon:||Escherichia coli plasmid pMB1 origin
Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4
Phage f1 origin
|Copy number:||Low copy number|
|Host range:||Escherichia coli
Saccharomyces cerevisiae; leu2(-)
|Further information:||The plasmid was constructed by replacing the VF2 fragment in p415-VF2 by the fragment encoding amino acids 1-172 of Cerulean using BspEI and XhoI.
This plasmid is meant for multicolor bimolecular fluorescence complementation in yeast, which allows the in vivo determination of multiple protein interactions at a time.
The plasmid encodes for Cerulean, which is the "bluest" cyan fluorescent variant of the A. victoria GFP protein.
The N-terminal fragment of the Cerulean coding sequence is preceded by a multiple cloning site.
The gene of interest should be cloned without its stop codon.
The plasmid has been partially sequenced by BCCM/GeneCorner, starting from the T3 and T7 promoter.
Other names of the plasmid are pNS1501 and p415-CFP-N for C term tagging.
|EMBL Accession number:||-|
|Latest sequence update:||06/01/2016|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: DraI, KpnI/PvuII, PstI and SacI/XhoI.|
|History of deposit:||The plasmid was deposited by Prof. Dr Nava Segev(1).
(1) Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, USA
|Plasmid reference:||Lipatova et al., Methods Mol. Biol. 1298 (2015), 107-116 [PMID: 25800836] [DOI: 10.1007/978-1-4939-2569-8_9]
|Restricted distribution:||- BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5α|
|Host reference:||Focus 8 (1986), 9
|Related host reference:||Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|p415-CFP-N for C (LMBP 9735) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr Nava Segev and was published in Lipatova et al., 2015.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.