Last data update: 24 January 2024 16:39 CET
Plasmid name: p415-YFP-N for C (LMBP 9736)
| New search | Print data sheet |
| Price category: | Cat. 2 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p9736.gb
(View with Genome Compiler) p9736.txt p9736.pdf |
| Sequence analysis results Genecorner: |
Sanger: 148925.fasta Sanger: 148926.fasta |
| Cloned DNA: |
Aequorea victoria green fluorescent protein DNA (GFP); enhanced yellow-green fluorescent variant (EYFP), yeast codon-optimised enhanced version of Venus (yEVenus), N-terminal fragment, C-terminal |
| Promoter: | Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1) Phage T3 promoter Phage T7 gene 10 promoter (T7g10); synthetic sequence Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
| Selection marker: | Ampicillin (amp) LEU2; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4 Phage f1 origin |
| Copy number: | Low copy number |
| Host range: | Escherichia coli Saccharomyces cerevisiae; leu2(-) |
| Parental clone: | p415-VF2; pKT103 |
| Further information: | The plasmid was constructed by replacing the VF2 fragment in p415-VF2 by the fragment encoding amino acids 1-172 of yEVenus, which was amplified from pKT103, using the BspEI and XhoI sites. This plasmid is meant for multicolor bimolecular fluorescence complementation in yeast, which allows the in vivo determination of multiple protein interactions at a time. The plasmid encodes a yeast codon-optimised enhanced version of Venus (yEVenus), which is the fastest maturing yellow variant of the A. victoria GFP protein. The N-terminal fragment of the yEVenus coding sequence is preceded by a multiple cloning site. The gene of interest should be cloned without its stop codon. Other names of the plasmid are pNS1502 and p415-YFP-N for C term tagging. |
| EMBL Accession number: | - |
| Latest sequence update: | 25/05/2018 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: DraI, KpnI/PvuII, PstI and SacI/XhoI. The plasmid has been partially sequenced by BCCM/GeneCorner, starting from the T3 and T7 promoter. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr Nava Segev(1). (1) Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, USA |
| Plasmid reference: | Lipatova et al., Methods Mol. Biol. 1298 (2015), 107-116 [PMID: 25800836] [DOI: 10.1007/978-1-4939-2569-8_9] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
p415-YFP-N for C (LMBP 9736) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr Nava Segev and was published in Lipatova et al., 2015. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.