Last data update: 31 October 2020 04:21 CET
Plasmid name: p415-YFP-N for C (LMBP 9736)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Cloned DNA:||Aequorea victoria green fluorescent protein DNA (GFP); enhanced yellow-green fluorescent variant (EYFP), yeast codon-optimised enhanced version of Venus (yEVenus), N-terminal fragment, C-terminal
|Promoter:||Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1)
Phage T3 promoter
Phage T7 gene 10 promoter (T7g10); synthetic sequence
Escherichia coli lac operon promoter
|Terminator:||Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)|
|Selection marker:||Ampicillin (amp)
|Replicon:||Escherichia coli plasmid pMB1 origin
Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4
Phage f1 origin
|Copy number:||Low copy number|
|Host range:||Escherichia coli
Saccharomyces cerevisiae; leu2(-)
|Parental clone:||p415-VF2; pKT103|
|Further information:||The plasmid was constructed by replacing the VF2 fragment in p415-VF2 by the fragment encoding amino acids 1-172 of yEVenus, which was amplified from pKT103, using the BspEI and XhoI sites.
This plasmid is meant for multicolor bimolecular fluorescence complementation in yeast, which allows the in vivo determination of multiple protein interactions at a time.
The plasmid encodes a yeast codon-optimised enhanced version of Venus (yEVenus), which is the fastest maturing yellow variant of the A. victoria GFP protein.
The N-terminal fragment of the yEVenus coding sequence is preceded by a multiple cloning site.
The gene of interest should be cloned without its stop codon.
The plasmid has been partially sequenced by BCCM/GeneCorner, starting from the T3 and T7 promoter.
Other names of the plasmid are pNS1502 and p415-YFP-N for C term tagging.
|EMBL Accession number:||-|
|Latest sequence update:||25/05/2018|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: DraI, KpnI/PvuII, PstI and SacI/XhoI.|
|History of deposit:||The plasmid was deposited by Prof. Dr Nava Segev(1).
(1) Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, USA
|Plasmid reference:||Lipatova et al., Methods Mol. Biol. 1298 (2015), 107-116 [PMID: 25800836] [DOI: 10.1007/978-1-4939-2569-8_9]
|Restricted distribution:||- BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5α|
|Host reference:||Focus 8 (1986), 9
|Related host reference:||Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|p415-YFP-N for C (LMBP 9736) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr Nava Segev and was published in Lipatova et al., 2015.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.