Last data update: 24 January 2024 16:39 CET
Plasmid name: p415GALLmBAX (LMBP 4575)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4575.gb
(View with Genome Compiler) p4575.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse Bcl2-associated X cDNA (Bax, BAX); α variant |
| Promoter: | Escherichia coli lac operon promoter Phage T3 promoter Phage T7 gene 10 promoter (T7g10); synthetic sequence Saccharomyces cerevisiae galactokinase promoter (GAL1); with deletion of one of the three UAS elements (GALL) |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
| Selection marker: | Ampicillin (amp) LEU2; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4 |
| Host range: | Escherichia coli Saccharomyces cerevisiae; leu2(-) |
| Parental clone: | p415GALL; pSCGAL1mBAX-L |
| Further information: | The plasmid was constructed by substituting the HindIII-XbaI fragment from p415GALL by the HindIII-XbaI fragment from pSCGAL1mBAX-L, containing the α variant of the mouse Bcl2-associated X cDNA (mBAX). p415GALLmBAX is a low-copy number shuttle vector for expression of the mouse Bax-α protein. The GALL promoter is a deletion variant of the Saccharomyces cerevisiae galactokinase promoter (GAL1), which lacks one of the three UAS elements required for full induction of the promoter by galactose. The GALL promoter is tightly repressed by glucose and is induced at a moderate level by galactose. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of the mBAX cDNA corresponds with the EMBL Nucleotide Sequence Database accession number L22472.1. Other name of the plasmid is p415GALLBAX. |
| EMBL Accession number: | L22472.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 07/03/2003 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BanII, BglI/HindII, HindIII/XbaI and XhoI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Contreras(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Reekmans et al., FEMS Yeast Res. 5 (2005), 711-725 [PMID: 15851100] [DOI: 10.1016/j.femsyr.2004.12.010] |
| Related plasmid reference: | Mumberg et al., Nucleic Acids Res. 22 (1994), 5767-5768 [PMID: 7838736] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
p415GALLmBAX (LMBP 4575) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Contreras and was published in Reekmans et al., 2005. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.