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GeneCorner plasmid details

Last data update: 30 October 2020 04:11 CET

Plasmid name: p416-CtSHS-M (LMBP 11198)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p11198.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Candida albicans SAT1 gene disruption cassette
Saccharomyces cerevisiae 2µ plasmid site-specific recombinase Flp cDNA (FLP1)
Promoter: Candida albicans elongation factor 1-alpha 2 promoter (TEF2)
Candida tropicalis alpha-glucosidase promoter (MAL2)
Candida tropicalis sulfate adenylyltransferase promoter (MET3)
Phage T7 gene 10 promoter (T7g10)
Escherichia coli lac operon promoter
Candida albicans actin promoter (ACT1)
Ribosome
binding site:
-
Terminator: Candida albicans ATP-dependent 6-phosphofructokinase subunit beta terminator (PFK2)
Candida albicans actin terminator (ACT1)
Selection marker: Saccharomyces cerevisiae URA3; auxotrophic
Ampicillin (amp)
Hygromycin (hyg)
Replicon: Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4
Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Candida tropicalis; ura3(-)
Saccharomyces cerevisiae; ura3(-)
Escherichia coli
Parental clone: p416-CtSHS
Further information: The plasmid was constructed by cloning the Candida tropicalis MET3 promoter, downstream of an FRT site, into the p416-SHS-TI vector, between the second SAT1 fragment and FRT site at the end of the gene disruption cassette.
The plasmid is intended for conditional gene expression in Candida tropicalis.
The nucleotide sequence of the plasmid corresponds with the EMBL Nucleotide Sequence Database accession number MF048873.1.
EMBL Accession number: MF048873.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 26/03/2019
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: The plasmid was deposited by Dr K. Ganesan(1). It was constructed by Dr S. Bijlani(1).
(1) CSIR Institute of Microbial Technology, Chandigarh, India
Plasmid reference: -
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

p416-CtSHS-M (LMBP 11198) is available at BCCM/GeneCorner. The plasmid was deposited by Dr K. Ganesan.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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