Last data update: 24 January 2024 16:39 CET
Plasmid name: p416-CtSHS-M (LMBP 11198)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p11198.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Candida albicans SAT1 gene disruption cassette Saccharomyces cerevisiae 2µ plasmid site-specific recombinase Flp cDNA (FLP1) |
| Promoter: | Candida albicans elongation factor 1-alpha 2 promoter (TEF2) Candida tropicalis alpha-glucosidase promoter (MAL2) Candida tropicalis sulfate adenylyltransferase promoter (MET3) Phage T7 gene 10 promoter (T7g10) Escherichia coli lac operon promoter Candida albicans actin promoter (ACT1) |
| Ribosome binding site: |
- |
| Terminator: | Candida albicans ATP-dependent 6-phosphofructokinase subunit beta terminator (PFK2) Candida albicans actin terminator (ACT1) |
| Selection marker: | Saccharomyces cerevisiae URA3; auxotrophic Ampicillin (amp) Hygromycin (hyg) |
| Replicon: | Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4 Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Candida tropicalis; ura3(-) Saccharomyces cerevisiae; ura3(-) Escherichia coli |
| Parental clone: | p416-CtSHS |
| Further information: | The plasmid was constructed by cloning the Candida tropicalis MET3 promoter, downstream of an FRT site, into the p416-SHS-TI vector, between the second SAT1 fragment and FRT site at the end of the gene disruption cassette. The plasmid is intended for conditional gene expression in Candida tropicalis. The nucleotide sequence of the plasmid corresponds with the EMBL Nucleotide Sequence Database accession number MF048873.1. |
| EMBL Accession number: | MF048873.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 26/03/2019 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr K. Ganesan(1). It was constructed by Dr S. Bijlani(1). (1) CSIR Institute of Microbial Technology, Chandigarh, India |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
p416-CtSHS-M (LMBP 11198) is available at BCCM/GeneCorner. The plasmid was deposited by Dr K. Ganesan. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.