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GeneCorner plasmid details

Last data update: 23 October 2020 04:27 CEST

Plasmid name: p416-HAH2 (LMBP 11188)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p11188.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Candida albicans gene disruption cassette
Promoter: Escherichia coli lac operon promoter
Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Candida albicans ARG4; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4
Host range: Escherichia coli
Candida albicans; arg4(-)
Saccharomyces cerevisiae; arg4(-)
Parental clone: pRS416
Further information: The plasmid was constructed by cloning a HIS1-ARG4-HIS1 disruption cassette, flanked by loxP sites, into the pRS416 vector.
The plasmid is meant for gene disruption or gene deletion in Candida albicans.
The nucleotide sequence of the plasmid corresponds with the EMBL Nucleotide Sequence Database accession number KF318042.1.
Other name of the plasmid is pFA-HAH2.
EMBL Accession number: KF318042.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 26/03/2019
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: The plasmid was deposited by Dr K. Ganesan(1). It was constructed by Dr S. Choudhury(1) and S. Malik(1).
(1) CSIR Institute of Microbial Technology, Chandigarh, India
Plasmid reference: -
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

p416-HAH2 (LMBP 11188) is available at BCCM/GeneCorner. The plasmid was deposited by Dr K. Ganesan.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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