Last data update: 24 January 2024 16:39 CET
Plasmid name: p416-HAH2-MET3 (LMBP 11190)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p11190.gb |
| Sequence analysis results Genecorner: |
NGS: h08-gc-dec2019.fasta |
| Cloned DNA: |
Candida albicans gene disruption cassette |
| Promoter: | Candida albicans sulfate adenylyltransferase promoter (MET3) Escherichia coli lac operon promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Candida albicans ARG4; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4 |
| Host range: | Candida albicans; arg4(-) Saccharomyces cerevisiae; arg4(-) Escherichia coli |
| Parental clone: | p416-HAH2 |
| Further information: | The plasmid was constructed by cloning the MET3 promoter downstream of the loxP site of the HAH2 cassette of p416-HAH2. This plasmid contains a HIS1-ARG4-HIS1 gene disruption cassette and is suitable for methionine repressible expression in Candida albicans. The nucleotide sequence of the plasmid corresponds with the EMBL Nucleotide Sequence Database accession number MF048860.1. |
| EMBL Accession number: | MF048860.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 26/03/2019 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr K. Ganesan(1). It was constructed by S. Malik(1) and H. Kaur(1). (1) CSIR Institute of Microbial Technology, Chandigarh, India |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
p416-HAH2-MET3 (LMBP 11190) is available at BCCM/GeneCorner. The plasmid was deposited by Dr K. Ganesan. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.