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GeneCorner plasmid details

Last data update: 01 December 2020 04:11 CET

Plasmid name: p416-HAH2-MET3 (LMBP 11190)

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Price category: Cat. 3 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p11190.gb
Sequence
analysis results
Genecorner:

NGS: h08-gc-dec2019.fasta

Cloned DNA: Candida albicans gene disruption cassette
Promoter: Candida albicans sulfate adenylyltransferase promoter (MET3)
Escherichia coli lac operon promoter
Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Candida albicans ARG4; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4
Host range: Candida albicans; arg4(-)
Saccharomyces cerevisiae; arg4(-)
Escherichia coli
Parental clone: p416-HAH2
Further information: The plasmid was constructed by cloning the MET3 promoter downstream of the loxP site of the HAH2 cassette of p416-HAH2.
This plasmid contains a HIS1-ARG4-HIS1 gene disruption cassette and is suitable for methionine repressible expression in Candida albicans.
The nucleotide sequence of the plasmid corresponds with the EMBL Nucleotide Sequence Database accession number MF048860.1.
EMBL Accession number: MF048860.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 26/03/2019
Authenticity test: This plasmid has been fully sequenced but the NGS sequence data still need to be implemented.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: The plasmid was deposited by Dr K. Ganesan(1). It was constructed by S. Malik(1) and H. Kaur(1).
(1) CSIR Institute of Microbial Technology, Chandigarh, India
Plasmid reference: -
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

p416-HAH2-MET3 (LMBP 11190) is available at BCCM/GeneCorner. The plasmid was deposited by Dr K. Ganesan.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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